A limited reduction of the disulfide bonds of bovine enterokinase (enteropeptidase, EC 3.4.21.9) was accomplished with 50 mM dithioerythritol, at pH 9.0, and at 4 degrees C. The conditions separated the heavy and light subunits quantitatively with improved reliability when compared to the conditions used previously (Savithri, H. S., and Light, A. (1980) Biochim. Biophys. Res. Commun, 94, 360-36...

Background: The SARS-CoV-2 pandemic is a global threat to humans and the world’s economy. Effective safe vaccines against this virus are essential control eradicate pandemic. currently applied carry spike-protein mRNA/cDNA. These go through several cellular processes in recipients for producing antigens. On contrary, RBD (receptor binding domain)-protein an antigen. ...

Three enzymes of intestinal origin-enterokinase, alkaline phosphatase, and sucrase-were released into the perfused small intestinal lumen of the rat upon intravenous injection of the gastrointestinal hormone cholecystokinin-pancreozymin (CCK-PZ). The presence of bile in the perfusion fluid greatly augmented this release. The results suggest that a combined mechanism of enzyme liberation due to ...

An enterokinase (Enteropeptidase, EC. 3.4.21.9) has been described in the pharate adult of Glossina mositans morsitans. The enzyme is present in pharate adults, 21 days after pupation. It activated commercial crystalline bovine trypsinogen to trypsin. It showed affinity for concanavalin A bound to sepharose and was reversibly sensitive to boiling at pH 6.0. The apparent molecular weight, as det...

INTRODUCTION Our objective was to investigate the effects of the administration of pancreatic homogenates, with or without enzymatic activation, to healthy animals regarding cytokine serum levels and the development of pulmonary distress. MATERIAL AND METHODS 106 male Wistar rats, divided into three groups, were studied: group A, intraperitoneal administration of homogenates activated with en...

According to their sugar recognition specificity, plant lectins are proposed as bioactive proteins with potential in cancer treatment and diagnosis. Helja is a mannose-specific jacalin-like lectin from sunflower which was shown to inhibit the growth of certain fungi. Here, we report its recombinant expression in a prokaryotic system and its activity in neurobalstoma cells. Helja coding sequence...

The production of pure protein is indispensable for many applications in life sciences, however protein purification protocols are difficult to establish, and the experimental procedures are usually tedious and time-consuming. Therefore, a number of tags were developed to which proteins of interest can be fused and subsequently purified by affinity chromatography. We report here on a novel lect...