The early endosomal protein Rab5 is highly expressed in tumor samples, although a causal relationship between Rab5 expression and cell transformation has not been established. Here, we report the functional effects of targeting endogenous Rab5 with specific shRNA sequences in different tumor cell lines. Rab5 down-regulation in B16-F10 cells decreased tumor formation by subcutaneous injection in...

Purpose of the study. An analysis indices free radical oxidation and respiration mitochondria heart cells in a malignant process presence diabetes mellitus chronic neurogenic pain experimental animals. Materials methods. The study included outbred female rats (n=32) ?57?L/6 mice (n=84). Experimental groups were: intact group 1 (n=8), control (n=8) with (DM), comparison standard subcutaneous tra...

The data center network is increasingly a cost, reliability and performance bottleneck for cloud computing. Although multi-tree topologies can provide scalable bandwidth and traditional routing algorithms can provide eventual fault tolerance, we argue that recovery speed can be dramatically improved through the co-design of the network topology, routing algorithm and failure detector. We create...

1 0 2 0 1 1 (a) Suppose a2 = (1, 2, 2) , a3 = (−2, 0, 1) , a4 = (0, 4, 5) , a5 = (0, 1, 1) . Compute the ranks of A and B. Explain why B can not be obtained from A by a finite number of elementary row operations. Solution: The first 3 columns of B are independent, so its column space has dimension 3, thus rank(B)=3. The second, third and fifth column of A are independent, so its column space ha...

We previously demonstrated that noncytolytic butano! extraction of B16 melanoma cells can increase the number of experimental lung métastases,and that brief incubation of the extracted cells with the extracted moieties reduces metastatic phenotype. This study examined the possibility that the extracted components are endogenous inhibitors of tumor cell surface-associated, degradative enzymes. ...

The role of cell surface glycoproteins in determining in vivo blood-borne arrest and survival characteristics of murine melanoma sublines of low (B16-F1) or high (B16-F10) potential to form experimental lung metastases after injection i.v. was assessed after inhibiting tumor cell protein glycosylation with tunicamycin. Incubation of B16-F1 or B16-F10 cells with 0.5 micrograms (or above) tunicam...

PURPOSE The most aggressive form of skin cancer is the malignant melanoma. Because of its high metastatic potential the early detection of primary melanoma tumors and metastases using non-invasive PET imaging determines the outcome of the disease. Previous studies have already shown that benzamide derivatives, such as procainamide (PCA) specifically bind to melanin pigment. The aim of this stud...

PURPOSE Tumor cell metastasis constitutes a major problem in the treatment of cancer. Because the cure rate of metastatic tumors is very low, new therapeutic approaches are needed. Heat shock protein 90 (HSP90) is a molecular chaperone that is recognized as a new target for the treatment of cancer. Here, we examine the value of a monoclonal antibody (mAb) against HSP90, mAb 4C5, as a potential ...

Breast cancer most frequently metastasizes to bone causing decreased quality of life and morbidity. Since current treatments are palliative, strategies to prevent bone metastases in breast cancer patients are required. There is substantial evidence indicating that high levels of nitric oxide (NO) suppress tumor growth and metastasis in vivo. We hypothesize that agents that produce high concentr...

BACKGROUND The 16 kDa heat shock protein (HSP) is an immuno-dominant antigen, used in diagnosis of infectious Mycobacterium tuberculosis (M.tb.) causing tuberculosis (TB). Its use in serum-based diagnostics is limited, but for the direct identification of M.tb. bacteria in sputum or cultures it may represent a useful tool. Recently, a broad set of twelve 16 kDa specific heavy chain llama antibo...