The binding of bacteria or bacterial products to host proteins of tissue extracellular matrix may be a mechanism of tissue adherence. We investigated interactions of the plasmid-encoded outer membrane protein YadA, which confers pathogenic functions on enteropathogenic yersiniae, with fibronectin. Attachment of YadA-positive and YadA-negative recombinant Yersinia enterocolitica strains to carti...

Analysis of plasma proteins avidly bound to T. pallidum surfaces revealed the ability of T. pallidum to acquire numerous host macromolecules. No acquisition was evident by the avirulent spirochete, T. phagedenis biotype Reiter. Western blotting technology using hyperimmune antifibronectin serum as a probe revealed the ability of virulent treponemes to avidly bind fibronectin from a complex medi...

Fibronectin (Fn) is an approximately 450 kDa glycoprotein that consists of 12 type I, 2 II, and 15–17 III modules. Fibrillation Fn important for tissue reconstitution wound healing. We previously reported Clostridium perfringens produces several Fn-binding proteins (Fbps), two which, FbpA FbpB, bind to III1-C (a fragment derived from the carboxyl-terminal two-thirds first-type module). Dermatop...

objective(s) staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. antibiotic therapy for vancomycin resistant s. aureus (vrsa) can not promise the eradication of infections. since adhesion is the major route of infections, adhesin based vaccine could suppress s. aureus infections. fibronectin binding protein a (fnbpa) and clumping factor a (clfa) ...

background lactoferrin is a multipurpose protein of the transferrin domestic. it is a spherical glycoprotein with a molecular weight of about 80 kda which is widely found in secretory discharges, such as milk, saliva, tear, and nasal secretions. lactoferrin is also found in secondary granules of polymorphonuclear leukocytes (pmn) and is released by some acinar cells. it also shows antibacterial...

Transmission of leptospirosis occurs through contact of mucous membranes and abraded skin with freshwater contaminated by pathogenic Leptospira spp. Exposure to physiological osmolarity induces leptospires to express high levels of the Lig surface proteins containing imperfect immunoglobulin-like repeats that are shared or differ between LigA and LigB. We report that osmotic induction of Lig is...

Fibronectin coating increases implant biocompatibility by enhancing surface endothelializationviaintegrin-mediated binding.

OBJECTIVE More than 70% of circulating homocysteine is disulfide-bonded to protein, but little is known about the specific proteins that bind homocysteine and their function as a consequence of homocysteine binding. METHODS AND RESULTS When human plasma was incubated with [(35)S]L-homocysteine, most of the homocysteine bound to albumin. However, additional homocysteine-binding proteins were d...

Staphylococcus aureus is an opportunistic bacterial pathogen responsible for a diverse spectrum of human diseases and a leading cause of nosocomial and community-acquired infections. Development of a vaccine against this pathogen is an important goal. The fibronectin binding protein A (FnBPA) of S. aureus is one of multifunctional 'microbial surface components recognizing adhesive matrix molecu...

Porphyromonas (Bacteroides) gingivalis W12 binds and degrades human plasma fibronectin. In the presence of the protease inhibitor N-alpha-p-tosyl-L-lysyl chloromethyl ketone, P. gingivalis cells accumulated substantial amounts of 125I-fibronectin as a function of incubation time. Fibronectin binding was specific, reversible, and saturable. The Kd for the reaction was estimated to be on the orde...