Tri-block poly (lactide) poly(ethylene glycol) poly(lactide) (PLA–PEG–PLA) copolymers are among the most attractive nano-carriers for gene delivery into mammalian cells, due to their biocompatibility and biodegradability properties. However, the low efficiency of the gene delivery by these copolymers is an obstacle to gene therapy. Here, we have investigated nanoparticles formulated using the p...

in order to improve the performance of irrigation networks, provide higher flexibility levels and improve water delivery methods, application of automatic control systems is suggested. in the coarse of the present study, a mathematical model of automatic pivot weir as based on pid control logic in combination with icss hydrodynamic model is developed. the developed model was tested under high f...

Gene therapy as a modern therapeutic approach has not yet advanced to a globally-approved therapeutic approach. Lack of adequate reliable gene delivery system seems to be one of the major reasons from the pharmaceutical biotechnology point of view. Main obstacles delaying successful application of human gene therapy are presented in this review. The unique advantages of non-biological gene carr...

We characterized a recently developed hyperactive piggyBac (pB) transposase enzyme [containing seven mutations (7pB)] for gene transfer in human cells in vitro and to somatic cells in mice in vivo. Despite a protein level expression similar to that of native pB, 7pB significantly increased the gene transfer efficiency of a neomycin resistance cassette transposon in both HEK293 and HeLa cultured...

This review deals with the layer-by-layer (LbL) assembly of polyelectrolyte multilayers of biopolymers, polypeptides (i.e., poly-l-lysine/poly-l-glutamic acid) and polysaccharides (i.e., chitosan/dextran sulphate/sodium alginate), onto thermo- and/or pH-responsive micro- and nano-gels such as those based on synthetic poly(N-isopropylacrylamide) (PNIPAM) and poly(acrylic acid) (PAA) or biodegrad...

introduction: vaccinology provides promising approaches for the control of various infectious diseases. among different strategies, dna vaccines offer attractive research opportunities for development of vaccines for induction of antigen-specific immunity owing to their stability, simplicity of delivery, safety and cost effectiveness. however, there is a need to increase their potency by the us...