Polyethylenimine (PEI)-DNA nanoparticles are used to invoke specific therapeutic response in dysfunctional cells. The efficacy of PEI gene delivery is partly attributed its buffering capacity, which enables endosomal/lysosomal escape based on the hypothesized “proton sponge effect”. However, hypothesis highly debated, and mechanistic picture endosomal remains unclear. In our previous work, prep...

To overcome the problems of gene and drug delivery, nanotechnology has gained interest in recent years. Nanosystems with different compositions and biological properties have been extensively investigated for drug and gene delivery applications. Nanotechnology in drug delivery has been manifested into nanoparticles that can have unique properties both in vitro and in vivo, especially in targete...

thermodynamic features related to preparation and use of self-assemblies formed between multilamellar and unilamellar zwitterionic liposomes and polynucleotides with various conformation and sizes are presented. the divalent metal cation or surfactant-induced adsorption, aggregation and adhesion between single- and double-stranded polyribonucleotides and phosphatidylcholine vesicles was followe...

objective(s): p-glycoprotein (p-gp) is an efflux protein, the overexpression of which has been associated with multidrug resistance in various cancers. although sirna delivery to reverse p-gp expression may be promising for sensitizing of tumor cells to cytotoxic drugs, the therapeutic use of sirna requires effective carriers that can deliver sirna intracellularly with minimal toxicity on targe...

Gene delivery is a promising technique that involves in vitro or in vivo introduction of exogenous genes into cells for experimental and therapeutic purposes. Successful gene delivery depends on the development of effective and safe delivery vectors. Two main delivery systems, viral and non-viral gene carriers, are currently deployed for gene therapy. While most current gene therapy clinical tr...