نتایج جستجو برای: hydrodynamic based gene delivery
تعداد نتایج: 4067033 فیلتر نتایج به سال:
objective(s): although viral vectors are considered efficient gene transfer agents, their board application has been limited by toxicity, immunogenicity, mutagenicity and small gene carrying capacity. non-viral vectors are safe but they suffer from low transfection efficiency. in the present study, polyallylamine (paa) in two molecular weights (15 and 65 kda) was modified by alkane derivatives ...
Objective(s): PEI based nanoparticle (NP) due to dual capabilities of proton sponge and DNA binding is known as powerful tool for nucleic acid delivery to cells. However, serious cytotoxicity and complicated conditions, which govern NPs properties and its interactions with cells practically, hindered achievement to high transfection efficiency. Here, we have tried to optimize the properties of ...
The Sleeping Beauty (SB) transposon system has been shown to enable long-term gene expression by integrating new sequences into host cell chromosomes. We found that the recently reported SB100x hyperactive transposase conferred a surprisingly high level of long-term expression after hydrodynamic delivery of luciferase-encoding reporter transposons in the mouse. We conducted dose-ranging studies...
Skeletal muscle is an interesting target for gene therapy. To achieve effective gene introduction in skeletal muscle, a hydrodynamic approach by intravenous injection of plasmid DNA (pDNA) with transient isolation of the limb has attracted attention. In this study, we demonstrated that polyplex nanomicelle, composed of poly(ethyleneglycol) (PEG)-block-polycation and pDNA, showed excellent capac...
objective(s): the introduction of nucleic acids into cells for therapeutic objectives is significantly hindered by the size and charge of these molecules and therefore requires efficient vectors that assist cellular uptake. for several years great efforts have been devoted to the study of development of recombinant vectors based on biological domains with potential applications in gene therapy....
abstract among synthetic carriers, dendrimers with the more flexible structure have attracted a great deal of researchers' attention in the field of gene delivery. followed by the promising results upon hydrophobic modification on polymeric structures in our laboratory, alkylcarboxylated poly (propylenimine)-based carriers were synthesized by nucleophilic substitution of amines with alkyl moiet...
conclusions eudragit coated nanoparticle of chitosan is an efficient choice for oral delivery of dna to upper part of gi tract. results the mean size and zeta potential of particles were 300 ± 4 nm and 14 ± 0.5 mv, respectively. encapsulation of this system was 89.6 ± 1.2%. dna release from batches was less than 12% at ph = 5.2 and more than 60% at ph = 6.8 with significant difference of p < 0....
In the present study, we have tested the ability of hydrodynamically delivered IL-2 cDNA to modulate the number and function of murine leukocyte subsets in different organs and in mice of different genetic backgrounds, and we have evaluated effects of this mode of gene delivery on established murine tumor metastases. Hydrodynamic administration of the IL-2 gene resulted in the rapid and transie...
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