One way to get an insight into the potential interaction between a pair of genes is to compare the phenotype of the double knockout organism to the phenotype we would expect if the genes were independent. However, since gene knockouts are expensive and time-consuming, we would like to be able to predict gene interactions. We used a dataset consisting of 352 yeast genes for which only a few doub...

Regulatory On-Off Minimization (ROOM) is a model for predicting the behavior of metabolic networks in response to gene knockouts. It is based on minimizing the number of significant flux changes (hence, on/off) with respect to the wild-type. ROOM outperforms a previously suggested model, Minimization Of Metabolic Adjustment (MOMA), whose minimization metric is Euclidian. Furthermore, ROOM shows...

We classify those triples (n, l,w) for which there exists a ‘knockout’ tournament for n players in which the winner always wins exactly w games and each loser loses exactly l games.

Human RNase H1 cleaves RNA only when the RNA is present in a DNA-RNA heteroduplex. Previous efforts to create RNase H1 knockout mice resulted in embryonic lethality1, but demonstrated that RNase H1 is required for mitochondrial function. We constructed viable constitutive hepatocyte liver specific RNase H1 knock out mice by coupling the Cre recombinase to an albumin promoter as albumin is not e...

Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the development of bilateral renal cysts which enlarge continuously, leading to compression adjacent intact nephrons. The growing lead a progressive decline in function. Cyst growth driven enhanced cell proliferation and chloride secretion into cyst lumen. Chloride believed occur mainly cAMP-activated cystic fibrosis trans...

ORP4L, a member of the Oxysterol-binding protein (OSBP) related-proteins (ORPs) family, has been shown crucial in cell survival. But its role in macrophage function and effect in atherosclerosis are not clear. The mouse lack of ApoE spontaneously develops atherosclerosis lesions on a high-fat diet, and the distribution of the atherosclerosis plaques is similar to human. Thus ApoE knockout mouse...