Background & Aim:Breast cancer is the second most common cancer in women after lung cancer. Given that the herbal ingredients are used for centuries to treat cancer,The aim of this study was to determine the cytotoxic effect of hydroalcholic extract from Thymus daenesisCelak on MCF-7 cancer cells line. Experimental: Breast cancer cells MCF-7 and natur...

AIM OF THE STUDY ATP-binding cassette super family G2 (ABCG2) is recognized as the key point of the cancer cells' resistance to chemotherapy drugs. But there have been rare reports about the relationship between ABCG2 and the invasion, migration and animal tumor formation abilities of cancer cells. We want to establish a recombinant lentiviral vector with ABCG2 overexpression, and study the vec...

We previously showed that S-adenosylmethionine-mediated hypermethylation of the PTEN promoter was important for the growth of tamoxifen-resistant MCF-7 (TAMR-MCF-7) cancer cells. Here, we found that the basal expression level of methionine adenosyltransferase 2A (MAT2A), a critical enzyme for the biosynthesis of S-adenosylmethionine, was up-regulated in TAMR-MCF-7 cells compared with control MC...

Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lin...

Aminobisphosphonates are drugs administered for the treatment of bone resorption. They can indirectly activate peripheral γδ T cells and render tumor cells susceptible to lysis by Vγ9Vδ2 T cells. We have investigated the molecules involved in conjugate formation and killing of aminobisphosphonate-treated MCF-7 breast tumor cells by Vγ9Vδ2 T cells. Lysis of aminobisphosphonate (Pamidronate and Z...

Oxidative damage to DNA is thought to play a significant role in mutagenesis, aging, and cancer. Sensitivity to oxidative DNA damage and DNA repair efficiency were examined using a series of human breast epithelial cell lines-MCF-10A, MCF-10AT, and MCF-10ATG3B-with progressively elevated Ras protein. Breast epithelial cells were treated with H2O2, in the absence and presence of the DNA-repair i...

Objective: To explore the reversal effects of triptolide (TP) on drug-resistance of adriamycin (Adriacin doxorubicin, ADR)-resistant human breast cancer cell lines (MCF-7/ADR) in vivo and in vitro. Methods: effects of triptolide and adriamycin on MCF-7 and MCF-7/ADR cell proliferation was measured by CCK-8, the reversal effect of triptolide on the drug-resistance of MCF-7/ADR cells was also mea...

Background and objectives: Because of the importance of breast cancer as a first cause of morbidity and mortality among females, also the limitation of chemotherapy and based on literature of the use of herbal medicines against cancer, the current study was aimed to determine the efficacy of Berbers vulgaris fruit ethanol extract against MCF-7 breast cancer cell line. ...

The human ATP-binding cassette superfamily G (White) member 2 (ABCG2) gene and its murine homologue breast cancer resistance protein 1 (Bcrp1) are recently described ATP-binding cassette transporters associated with drug resistance in tumor cell lines, including the MCF-7 cell line, selected for its resistance to mitoxantrone (MCF-7/MitoR). Infection of MCF-7 cells with the retroviral vector co...