STUDY DESIGN Posterolateral lumbar transverse process fusion was carried out using cultured mesenchymal stem cells with or without bone morphogenetic protein (BMP) and basic fibroblast growth factor (FGF). OBJECTIVES To determine the ability of BMP and basic FGF to enhance the efficacy of bone marrow-derived mesenchymal stem cells in lumbar arthrodesis. SUMMARY OF BACKGROUND DATA Our previo...

Background: It is still controversial that the stem cells isolated from human dental pulp meets the criteria for mesenchymal stem cells (MSCs). The aim of the present study was to examine whether or not they are MSCs, or are distinct stem cells population residing in tooth pulp. Methods: Adherent fibroblastic cells in the culture of pulp tissue from human third molars were propagated through se...

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research about potential use of stem cells for the development of germ line cells in vitro had been challenged. in the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. the cells were purificated based on the expression on the cell surface of a protein. this protein is not present in normal cells of ...

experimental and clinical studies have shown that intracoronary transplantation of autologous bone marrow mesenchymal stem cells (bmscs) has resulted in regenerated infarcted myocardium and improved left ventricular (lv) function.the aim of this pilot study was to assess the benefical effectsof intracoronary transplantation of bmsc in patients with oldmyocardial infarction (omi). autologous bms...

conclusions the results of this study showed that the meticulous microsurgical nerve repair, which was performed using the vein tubulization induced significantly better sciatic nerve regeneration. however, the addition of bone marrow mesenchymal stem cell to vein conduit failed to promote any significant changes in regeneration outcome. results all animals tolerated the surgical procedures and...

multiple sclerosis is an inflammatory disease of the central nervous system in which t cells experience a second phase of activation, which ultimately leads to axonal demyelination and neurological disability. the recent advances in stem cell therapies may serve as potential treatments for neurological disorders. there are broad types of stem cells such as neural, embryonic, mesenchymal and hem...

BACKGROUND Bone marrow mesenchymal stem cells support proliferation and differentiation of hematopoietic progenitor cells in vitro. Since these cells constitute a rare subset of bone marrow cells, mesenchymal stem cell preparations for clinical purposes require a preparative step of ex vivo multiplication. The aim of our study was to analyze the influence of culture duration on mesenchymal stem...

human mesenchymal stem cell (msc) can be isolated from bone marrow (bm) and differentiated into multiple lineages. these properties make them promising tools in cell and gene therapy. up to now, no definite therapeutic intervention for late stages of multiple sclerosis (ms) has been found. we decided to inject ms patients with autologous expanded msc. five patients participated in this ongoing ...

Background: In recent decade, human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) provide enormous potential for appropriate cell therapy, but they have limited growth potential and cease to proliferate due to cellular senescence, so providing a strategy for increasing the stem cell survival is necessary.  Methods: In this investigation, MSCs characterized by flow cytome...