background: the aim of this study was to assess effects of cysteamine as an anti-oxidant on rate of in vitro maturation of oocyte and determination of its effects on spindle size and shape. methods: pre-mature mice were primed with pregnant mare stimulating gonadotrophin (pmsg) and germinal vesicle (gv) stage oocytes were obtained 48 h after. the oocytes were cultured in tissue culture medium (...

this work is conducted to obtain mechanical properties of microtubule. for this aim, interaction energy in alpha-beta, beta-alpha, alpha-alpha, and beta-beta dimers was calculated using the molecular dynamic simulation. force-distance diagrams for these dimers were obtained using the relation between potential energy and force. afterwards, instead of each tubulin, one sphere with 55 kda weight ...

In human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. The sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. The objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...

Neuronal migration and axon growth guidance require precise control of microtubule dynamics microtubule-based cargo transport. TUBB3 encodes the neuronal-specific β-tubulin isotype III, TUBB3, a component neuronal microtubules expressed throughout life central peripheral neurons. Human pathogenic missense variants result in altered function cause errors either cranial and, to lesser extent, axo...

Catharanthus roseus (L.) G.Don is a plant which produces anticancer and anti-mitotic indole alkaloids. Colchicine is an anti-mitotic drug. Anti-mitotic effects of Vinca herbacea Waldst. & Kit. indole alkaloids is unknown. The study were evaluated the antimitotic effect of alkaloids of V. herbacea, Catharanthus roseus andcolchicineon mitosis and microtubule arrangement of human blood cells. In t...

background: routine oocytes cryopreservation remained an elusive technique in the wide ranges of available assisted reproductive technologies. the microtubules of oocytes are vulnerable to cryoprotectants and thermal change during cryopreservation. objective: the effects of a vitrification protocol were investigated on the spindle and chromosome configurations of mice oocytes cryopreserved at t...

in human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. the sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. the objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...

•SBTub3 photocontrols microtubule dynamics, organization, and dependent processes •Microtubule photocontrol is cell sub-cellularly precise temporally reversible •SBT orthogonal to GFP/YFP imaging SBTs are metabolically stable •The SBT scaffold promising for photopharmaceuticals other protein targets Optically controlled chemical reagents, termed “photopharmaceuticals,” powerful tools spatiotemp...

The last two decades have witnessed a tremendous increase in cell biology data. Not least is this true for studies of the dynamic organization microfilament and microtubule systems animal cells where analyses molecular components their interaction patterns deepened our understanding these complex force-generating machineries. Previous observations cross-talk between now led to realization exist...

We systematically measure plus-end dynamics in large microtubule asters and find evidence for spatial regulation of depolymerization rate.