Phage PRD1 and adenovirus share a number of structural and functional similarities, one of which is the vertex organization at the fivefold-symmetry positions. We developed an in vitro mutagenesis system for the linear PRD1 genome in order to make targeted mutations. The role of protein P5 in the vertex structure was examined by this method. Mutation in gene V revealed that protein P5 is essent...

Insulin receptor substrate (IRS)-2 is structurally and functionally similar to IRS-1. Indeed, stimulation with insulin or insulin-like growth factor I led to the rapid tyrosine phosphorylation of both IRS-1 and IRS-2, which in turn activated phosphatidylinositol (PI) 3-kinase in L6 cells and rat skeletal muscle. However, IRS-2 was rapidly dephosphorylated (3-10 min after the addition of insulin...

The computational complexity of the Vertex Coloring problem is known for all hereditary classes of graphs defined by forbidding two connected five-vertex induced subgraphs, except for seven cases. We prove the polynomial-time solvability of four of these problems: for (P5, dart)-free graphs, (P5, banner)-free graphs, (P5, bull)-free graphs, and (fork, bull)-free graphs.

A graph is (P5,gem)-free, when it does not contain P5 (an induced path with five vertices) or a gem (a graph formed by making an universal vertex adjacent to each of the four vertices of the induced path P4) as an induced subgraph. We present O(n2) time recognition algorithms for chordal gem-free and for (P5,gem)free graphs. Using a characterization of (P5,gem)-free graphs by their prime graphs...

This paper focuses on the political economy of policy reform in post-socialist countries. It investigates the impact of institutions on economic transformation processes and seeks to develop and empirically test a concept of governance that will strengthen policy reforms and make them sustainable. By arguing that effective policy reforms require an adequate politico-institutional foundation, th...

During chemotactic signaling by Escherichia coli, autophosphorylation of the histidine kinase CheA is coupled to chemoreceptor control by the CheW protein, which interacts with the C-terminal P5 domain of CheA. To identify P5 determinants important for CheW binding and receptor coupling control, we isolated and characterized a series of P5 missense mutants. The mutants fell into four phenotypic...

During adeno-associated virus (AAV) type 2 productive infections, the p19 promoter of AAV is activated by the AAV Rep78 and Rep68 proteins. Rep-induced activation of p19 depends on the presence of one of several redundant Rep binding elements (RBEs) within the p5 promoter or within the terminal repeats (TR). In the absence of the TR, the p5 RBE and the p19 Sp1 site at position -50 are essential...

We present a protocol for anonymous communication over the Internet. Our protocol, called P5 (Peerto-Peer Personal Privacy Protocol) provides sender-, receiver-, and sender-receiver anonymity. P5 is designed to be implemented over the current Internet protocols, and does not require any special infrastructure support. A novel feature of P5 is that it allows individual participants to trade-off ...

Signal transduction by insulin and IGF-1, several interleukins (IL-2, IL-4, IL-9, IL-13), interferons, GH, and other cytokines involves IRS proteins, which link the receptors for these factors to signaling molecules with Src homology-2 domains (SH2-proteins). We recently reported the amino acid sequence of murine IRS-2; in order to examine a potential genetic role for this molecule in disease, ...