This paper describes a new method o5 plasmid DNA purification which is fast and reliable enough for most purposes in recombinant DNA technology. The present method does not require the use of toxic chemicals such as phenol or e th id ium bromide, costly ultracentri /ugation procedures or other processes which can modify the supercoiled structure of the plasmids, such as adsorption on glass fibe...

A bacterial plasmid is a species of nonessential extrachromosomal deoxyribonucleic acid (DNA) that replicates autonomously as a stable component of the cell's genome. Naturally occurring plasmids range in size from one to several hundred kilobases and in copy number from one to several hundred per cell. Copy number is a fixed characteristic of any plasmid under constant conditions and is contro...

Postsegregational killing (PSK) systems consist of a tightly linked toxin-antitoxin pair. Antitoxin must be continually produced to prevent the longer lived toxin from killing the cell. PSK systems on plasmids are widely believed to benefit the plasmid by ensuring its stable vertical inheritance. However, experimental tests of this "stability" hypothesis were not consistent with its predictions...

Since the discovery of plasmid, various methods have been developed to isolate plasmid DNA. All the methods have one common and important target of isolating plasmid DNA of high quality and quantity in less time. These methods are not completely safe because of use of toxic chemicals compounds. The developed protocol for plasmid extraction is based on the alkaline lysis method of plasmid prepar...

background: dna vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. bacteria of the genus brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. methods: to evaluate the use of a dna immunization strategy for protection against brucellosis, a plasmi...

Variovorax paradoxus VAI-C was isolated due to its ability utilize acyl-homoserine lactones (AHLs) as the sole source of carbon, energy, and nitrogen. Here, we present a hybrid assembly V. genome sequence, consisting primary chromosome, secondary chromid, plasmid.

Synthetic linear SARS-CoV-2 RNA fragments (ssRNA) and a double-stranded DNA plasmid harbouring the nucleocapsid (N) gene are evaluated in four different VTM for up to 10 freeze–thaw cycles by qRT-PCR following magnetic bead extraction.

OBJECTIVES To analyse the in vitro transfer of the qnrA1 gene by a 50 kb (pSZ50) self-transferable plasmid that derives from a 300 kb plasmid (pSZ300) and to determine the complete nucleotide sequence of plasmid pSZ50. METHODS Extended-spectrum β-lactamase (ESBL) and plasmid-mediated quinolone resistance (PMQR) genes of an Escherichia coli clinical isolate were analysed. Plasmid analysis incl...

Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (retaining a replication competent plasmid) and to investigate the plasticity of the plasmid. A recently developed, plasmid-based transformation prot...