The study aimed to investigate the occurrence of carbapenem resistant E. coli and P. aeruginosa in apparently healthy, captive blackbucks leopards India. Faecal samples (n = 7) were processed isolate (CRE) (CRP). Forty (leopards n 26; blackbuck 14) two (blackbuck 2) isolated from faecal 14). Eleven isolates recovered, which 10 CRE one was CRP. minimum inhibitory concentration (MIC) determined f...

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...

Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verifica...

background: protozoa related to trypanosome family including leishmania , synthesize enzymes to escape from drug therapy. one of them is ptr1 that its enzymatic activity is similar to dihydrofolate reductase (dhfr). dihydrofolate reductase - thymidylate synthase has a major role in dna synthesis, if it is inhibited, the result would be the death of parasite. since ptr1 activity is similar to dh...

recent technological improvements have extended the application range of dielectric permittivity biomass measurements to on-line monitoring of physiological changes during bacterial fermentations. in an industrial fermentation of bacillus thuringiensis, it is important to verify the intactness of bacterial plasmid content in all steps of culture. changes in certain plasmid resident properties o...

in this essay, the heavy metal resistance patterns of bacterial strains isolated from industrial wastewater, domestic wastewater and various parts of the wastewater treatment system of arak city have been studied. 28 intestinal bacterial strains were screened and identified as klebsiella, escherichia coli, citrobacter and enterobacter species biochemical methods. the minimum inhibitory concentr...

Methicillin-resistant Staphylococcus aureus (MRSA) has been listed by the World Health Organization as a priority antibiotic-resistant nosocomial pathogen in urgent need of new antimicrobials [1]. Plasmids are key factor pathology and epidemiology MRSA isolates play important roles their evolution. In our on-going efforts to better understand genetic background molecular from Terengganu, Malays...

Mutations in the gene encoding topoisomerase I of Escherichia coli were tested for their effect on plasmid recombination. Recombination was decreased 1,000-fold at 30 and 37 degrees C and occurred at approximately wild-type frequencies at 42 degrees C. The suppression of topA mutations at 42 degrees C did not appear to be a result of increased topoisomerase I activity at 42 degrees C.

Here we report the results of second interim analysis a randomized prospective multi-center clinical study aimed to evaluate safety and efficacy p62/SQTM1-encoding plasmid applied as an adjuvant chemotherapy in patients with advanced platinum-resistant ovarian cancer. P62 encoding acts classic anti-cancer DNA vaccine it also lowers chronic inflammation thus rendering tumor cells more susceptibl...