Since Engelmann’s (1871) demonstration of increased phosphorus excretion in urine secreted during muscular exercise, there have been many attempts to show that the increase originated in the muscles. Muscle phosphorus compounds remain obscure, and it has only been during the last 10 years that the inorganic phosphorus of tissues could be conveniently determined. During this period, tissue inorg...

Anacker, R. L. (Rocky Mountain Laboratory, Hamilton, Mont.), W. T. Haskins, D. B. Lackman, E. Ribi, and E. G. Pickens. Conversion of the phase I antigen of Coxiella burnetii to hapten by phenol treatment. J. Bacteriol. 85:1165-1170. 1963.-Trichloroacetic acid extracts of Coxiella burnetii are converted to hapten by treatment with phenol. Such extracts react, like the original trichloroacetic ac...

Mutants of Bacillus thuringiensis lacking either beta-exotoxin or gamma-endotoxin were compared for their virulence using pupae of a giant silk moth. Known doses of viable log-phase bacteria were injected, and the response was followed as the number of viable bacteria in the hemolymph. The results obtained imply that, in the system used, neither the beta-exotoxin nor the gamma-endotoxin and the...

In this study, four protein extraction protocols from Mytilus galloprovincialis were evaluated with the aim to identify the most practical, efficient and reproducible method. Four extraction protocols frequently used for mussels and organic matrices were selected and compared. The methods were based on the use of: i) TRIzol reagent; ii) Lysis buffer; iii) phenylmethanesulfonyl fluoride; iv) tri...

Approximately 15 ml. of venous blood were withdrawn and expressed into a chilled tube containing 0.30 ml. (300 U.S.P. units) of sodium heparin solution. After thorough but gentle stirring, 10.0 ml. were transferred by Folin pipette to 10 ml. of ice cold 10 per cent trichloroacetic acid. The mixture was thoroughly homogenized with a heavy stirring rod and centrifuged in the cold. The supernatant...

Introduction: Solar lentigo is local and benign proliferation of melanocytes which is common in old individuals and creates a major concern considering beauty. Despite several and somehow expensive treatments such as laser and cryotherapy, chemical peeling with Trichloroacetic Acid (TCA) has recently attracted attentions considering its low cost and availability. The present study was des...

A simplified scheme of analysis is presented for the determination of free and esterified cholesterol, the phospholipids, and the neutral fats (triglycerides) of blood serum. The hydroxamic acid reaction is used to determine total esterified fatty acids. The phospholipids are determined after acid digestion of a trichloroacetic acid precipitate of protein-phospholipid, and both total and free c...