In addition to neuritic changes and amyloid deposits, neuronal and glial cell apoptosis is an important pathological feature of Alzheimer's disease (AD). Several factors have been postulated as causes or triggers of cellular apoptotic change. This study focused on a quantifiable relationship between phosphorylation sites of tau protein in the neurofibrillary tangles (NFT) and neuronal apoptosis...

her, J.J. McCormick, G.P. Taylor and R.J. Klebe. 1998. Overview of matrix metalloproteinase expression in transformed cells. Matrix Biol. 16:483-496. 3.Hill, H.Z., C. Huselton, B. Pilas and G.J. Hill. 1987. Ability of melanins to protect against the radiolysis of thymine and thymidine. Pigment Cell Res. 1:81-86. 4.Kreader, C.A. 1996. Relief of amplification inhibition in PCR with bovine serum a...

The aim of the present study was to evaluate the distribution of apoptotic neurons in the nuclei of the brainstem. Apoptosis was studied by the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay, in human brainstems taken at autopsy from 15 adults (age range: 25-58 years). The nuclei examined included the hypoglossal nucleus (XII), dorsal motor nucleus of the va...

The aim of this study was to clarify whether myocardial cells with DNA fragmentation induced by ischemia show increased plasma-membrane permeability (PMP), focusing on the early lesions of acute myocardial ischemia (AMI). Rat AMI was induced by a permanent occlusion of the left coronary artery. The TUNEL method was used for the demonstration of DNA fragmentation, and lanthanum ions (La) were em...

The extent to which programmed cell death is the fate of proliferative, rather than post-mitotic, cells remains controversial, but a preponderance of evidence suggests that at least some cells within the brain's proliferative zone die during mammalian brain development. One major unresolved question is the extent to which cell death in the proliferative zone is spatiotemporally patterned. In or...

We examined the temporospatial pattern of naturally occurring apoptosis in chick embryos to five days of incubation (H.H. stages 1-25; Hamburger and Hamilton, 1951) using TUNEL labeling. The initial TUNEL-positive structure was the embryonic shield at stage 1. Apoptotic cells became ubiquitously present within embryos by stage 3, which is early in gastrulation. Until stage 6, TUNEL-positive cel...

BACKGROUND In this study we present the physiological changes observed in ejaculated spermatozoa of normospermic men after exposure to hydrogen peroxide (H(2)O(2)) or gamma irradiation. METHODS Motility changes as well as membrane and DNA-damage were determined in spermatozoa after incubation with 25 micromol/l of H(2)O(2) during increasing intervals of time (0--60 min and after 24 h) or afte...