We have shown previously that the Ca channel b3 subunit is capable of modulating tonic G-protein inhibition of a1A and a1B Ca channels expressed in oocytes. Here we determine the modulatory effect of the Ca channel b3 subunit on M2 muscarinic receptor-activated G-protein inhibition and whether the b3 subunit modulates the G-protein sensitivity of a1A and a1B currents equivalently. To compare th...

Despite the high level of symmetry between PsaA and PsaB polypeptides in Photosystem I, some amino acids pairs are strikingly different, such as PsaA-Gly693 PsaB-Trp673, which located near a cluster 11 water molecules A1A A1B quinones FX iron-sulfur cluster. In this work, we changed PsaB-Trp673 to PsaB-Phe673 Synechocystis sp. PCC 6803. The variant contains ~ 85% wild-type (WT) levels I but is ...

To investigate a1B-adrenoceptor function, we developed a phosphorothioate antisense oligodeoxynucleotide (AO) to inhibit the expression of the a1B-adrenoceptor subtype in DDT1 MF2 cells. We measured the cellular uptake of the AO and its effect on a1B-adrenoceptor mRNA expression, protein density, and coupling to phospholipase C. Cells treated with either a control oligodeoxynucleotide (CO) or m...

Kirsten R. McDonald, Neva J. Fudge, Janine P. Woodrow, James K. Friel, Ana O. Hoff, Robert F. Gagel, and Christopher S. Kovacs Faculty of Medicine Endocrinology, Memorial University of Newfoundland, St. John’s, Newfoundland A1B 3V6; Department of Biochemistry and Pediatrics, Memorial University of Newfoundland, St. John’s, Newfoundland A1B 3X9, Canada; and University of Texas MD Anderson Cancer...

Selective inactivation of a1B-adrenoceptor (AR) by the sitedirected alkylating agent chlorethylclonidine (CEC) has been used as one of major pharmacological criteria to subclassify a1-AR; however, the mechanism for the differential CEC sensitivity of the two subtypes is uncertain, and the extent of CEC inactivation varies depending on the treatment employed. In this study, we examined the corre...

The structural basis of Ca channel inhibition by G proteins has received considerable attention recently, and multiple regions on Ca channels that interact with G protein subunits have been identified. We have demonstrated previously that a region extending from the N terminus to the I/II loop of the Ca channel is involved in determining the differences between a1B and a1E Ca channels with resp...

The study aimed to find out possible changes in climatic data (temperature and rainfall) from the regional climate model viz. PRECIS(Providing Regional Climates for Impact Studies)under different SRES scenarios (A1B, A2 B2 scenario) by mid (2021-2050) end (2071-2100) century at six locations of Punjab representing agroclimatic zones their impact on maize yield using crop growth simulation model...

Two subspecies of Francisella tularensis are recognized: F. tularensis subsp. tularensis (type A) and F. tularensis subsp. holartica (type B). Type A has been subdivided further into A1a, A1b, and A2, which differ geographically and clinically. The aim of this work was to determine whether or not differences among subspecies and clades translate into distinct ecological niches. We used 223 isol...

Alternative Mechanisms Rationale/Arguments/ Assumptions Connected Literature/Theory Resulting (Possible) Effect Support for/Against A1. Based on WTP and Exposure Theory A1a Light user segments (who are likely to have low WTP) are more likely to reduce (or even discontinue in extreme cases) their consumption of NYT content after the paywall implementation. Utility theory — WTP (Danaher 2002) Jux...

We investigated the expression of a1-adrenergic receptor subtypes in intact human peripheral blood lymphocytes using reverse transcription–polymerase chain reaction (RT-PCR) and radioligand binding assay techniques combined with antibodies against the three subtypes of a1-adrenergic receptors (a1A, a1B, and a1D). RT-PCR amplified in peripheral blood lymphocytes a 348-bp a1A-adrenergic receptor ...