The genetic diversity of 33 Nigerian Helicobacter pylori isolates were studied using RAPD, PCR-RFLP and Southern blot analysis of ureA or ureCD gene probes. RAPD was able to distinguish the following number of isolates using the primers 3880: 5'-AAGAGCCCGT-3' (28), 3881 :5'-AACGCGCAAC-3' (33) and OPH8 :5'-GAAACACCCC-3' (25). Southern blot analysis using the ureCD probe was also able to distingu...

The aim of this work was to study the genetic diversity among flue-cured tobacco cultivars. RAPD and AFLP analyses were used to assess the genetic similarity among selected accessions of flue-cured tobacco. Seventy eight RAPD and 154 AFLP polymorphic bands were obtained and used to assess the genetic diversity among 28 tobacco accessions. The cultivar relationships were estimated through the cl...

The random amplified polymorphic DNA (RAPD) assay was used to assess the level of DNA damage in various exposed and unexposed Culex quinquefasciatus larvae to acetone and chloroform extracts of Curcuma longa and Melia azedarach at different concentrations (6.25, 12.5 and 25 ppm). This is the first report of an analysis of genomic alterations in plant extracts-treated mosquito larvae using RAPD-...

In this paper, nine geographical isolates of Schistosoma mansoni (three from Egypt, three from Saudi Arabia and 3 from Puerto Rico) were studied at the genotype level by RAPD analysis with two arbitrary primers. The genetic distance was measured by the percentage of unshared bands. The RAPD results showed that the Egyptian strains were closely related to Saudi strains but Puerto Rico strains cl...

We evaluated the ability of randomly amplified polymorphic DNA (RAPD) to type citrinin-producing Penicillium citrinum (P. citrinum) strains recovered from the forest's air in northern Iran. A total of 12 P. citrinum strains (P1-P12) were characterised by citrinin production and random amplification of polymorphic DNA (RAPD) technique. All the strains produced citrinin with levels ranging from 1...

Ten isolates of Paracoccidioides brasiliensis were examined for differences in virulence in outbred mice intravenously inoculated with the fungus, associated with mycelial morphology, and genetic patterns measured by random amplified polymorphic DNA (RAPD). Virulence was evaluated by viable yeast cell recovery from lungs and demonstration of histopathologic lesions in different organs. The resu...

Karnal bunt of wheat, caused by Tilletia indica Mitra is an important disease prevalent in several countries. The pathogen due to its heterothallic nature shows high variability. In the present study, genetic relationships among ten isolates of T.indica collected from different locations of India and 15 monosporidial lines raised from these isolates were investigated by using 34 RAPD and 28 ISS...

We compared the robustness and discriminatory power of the enterobacterial repetitive intergenic consensus (ERIC) and random amplified polymorphic DNA (RAPD) fingerprinting methods for detecting cases of mixed Helicobacter pylori infection in Peruvian shantytown residents. H. pylori isolates from 63 participants were cultured, and five single colonies and a pool of additional colonies from each...

The spore-forming bacterium Bacillus licheniformis is a common contaminant of milk and milk products. Strains of this species isolated from dairy products can be differentiated into three major groups, namely, G, F1, and F2, using random amplification of polymorphic DNA (RAPD) analysis; however, little is known about the genomic differences between these groups and the identity of the fragments...

Maize landraces derived from tropical germplasm represent an important source of genetic variability, which is currently poorly understood and under-exploited by Brazilian crop breeding programs. The aims of our study were to a) estimate the genetic diversity across 48 varieties of maize landraces cultivated at different locations in the States of Rio Grande do Sul (RS) and Paraná (PR) by ...