The aim of this study was to apply RAPD technique to analyze the genetic variability among the Iranian CA-MRSA isolates.The RAPD amplification was implemented on 25 strains isolated from the anterior nares of 410 healthy children using four randomly selected oligonucleotide primers from the stocks available in our laboratory, including the primers 1254, GE6, OLP6 and OLP13 from our stock. The a...

Chlorophytum borivilianum is a traditional medicinal plant distributed throughout the trop‐ ics and subtropics. In the present investigation, RAPD and ISSR analyses were used to assess the genetic diversity among 21 accessions collected from different geographical regions of India using 20 RAPD primers and 6 ISSR primers. RAPD and ISSR primers revealed 92.26% and 82.76% polymorphism, respective...

Two types of molecular markers, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were assayed to determine the genetic diversity of 46 barley accessions, including 27 landraces of H. vulgare ssp vulgare (HV), 6 accessions of H. vulgare ssp. spontaneum (HS) and 13 accessions of H. vulgare ssp. agriocrithon (HA), from west China. A high level of polymorphism was fo...

PURPOSE The aim of this study was to compare the asymmetrical light reflex of the control subjects and patients with optic nerve disease and to evaluate the relationships among the relative afferent pupillary defect (RAPD), visual acuity (VA), central critical fusion frequency (CFF), ganglion cell complex thickness (GCCT), and circumpapillary retinal nerve fiber layer thickness (cpRNFLT) using ...

The lethal gene 'Luteus-Pa' is found in cacao genotypes (Theobroma cacao) of the Parinari (Pa) series, from Peru. Seedlings affected by this gene have yellowing leaves and subsequently die. We mapped this gene based on microsatellite markers and RAPDs, in order to elucidate the inheritance of 'Luteus-Pa' and investigate possible lethal mechanisms. DNA samples of genitors were amplified with 87 ...

In the present study, 20 sandalwood (Santalum album L.) genotypes were characterized using RAPD, ISSR and SSR markers. Twenty-five RAPD and twenty-one ISSR primers that generated clear and reproducible banding patterns amplified 225 and 208 bands, respectively, among 20 sandalwood genotypes. Out of 225, 181 (83.13 %) RAPD bands were polymorphic while out of 208, 156 (75.77 %) ISSR bands were po...

Bunias orientalis and Erigeron annuus are two alien herbal plant species invading native ecosystems in Lithuania. Both species have similar introduction and invasion history but differ in their life history traits and strategies of reproduction. We used random amplified polymorphic DNA (RAPD) data to compare the genetic diversity in invasive populations of these species. 42 RAPD bands were iden...

PURPOSE To evaluate the validity of different methods of relative afferent pupillary defect (RAPD) detection and assess the usefulness of detecting an RAPD in glaucoma patients. METHODS For this prospective observational study, we enrolled 70 of 153 consecutive glaucoma patients coming for examination. Exclusion criteria were cloudy corneas, recent surgery, fixed pupils, and other diseases pr...

Molecular markers are used in plant science a variety of ways. The method molecular genome marking based on RAPD-PCR makes it possible to determine the genetic status populations and establish intrapopulation relationships. In this work, we studied polymorphism dandelion Taraxacum officionale using RAPD markers. Five random amplified polymorphic DNA (RAPD) were diversity relationship between tw...

This study reports the genetic variations between populations of the red palm weevil, Rhynchophorus ferrugineus in the UAE area. To test if the red palm weevil population in the UAE belongs to the same genetic group; the randomly amplified polymorphic DNA (RAPD) technique was used to assay seven populations of the insect from different locations. RAPD primer pairs detected a total 216 RAPD frag...