high frequency of icaad, clumping factors a/b, fib and eno genes in staphylococcus aureus species isolated from wounds in tehran, iran during 2012-2013


abdolmajid ghasemian department of bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran; department of bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran. tel: +98-92182883870

shahin najar peerayeh department of bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran

bita bakhshi department of bacteriology, faculty of medical sciences, tarbiat modares university, tehran, ir iran

mohsen mirzaee department of laboratory sciences, borujerd branch, islamic azad university, borujerd, ir iran


materials and methods this definitive and cross-sectional study was conducted in tehran, iran. a total of 15 s. aureus isolates were collected from wound samples with sterile swabs and were identified by the conventional diagnostic tests. the antibiotic susceptibility pattern was performed according to the clinical and laboratory standards institute (clsi, 2012) protocol. the meca gene, staphylococcal cassette chromosome mec (sccmec) types, agr specific groups and biofilm related genes were detected by polymerase chain reaction (pcr) assay. results the majority of the isolates were resistant to amoxicillin, tetracycline and erythromycin, but all were susceptible to vancomycin and linezolid. eight (40%) isolates were methicillin resistant and the meca gene was detected in these isolates. the majority of (90%) of mrsa harbored sccmec type iii, and two isolates harbored type v. the majority (70%, n = 14) of the isolates belonged to agr i, followed by agr ii (15%, n = 3), agr iv (10%, n = 2) and agr iii (5%, n = 1). the frequencies of clfab, fnbab, fib, eno, cna, ebps and bbp genes were 100%, 100%, 65% (n = 13), 55% (n = 11), 70% (n = 14), 70% (n = 14), 55% (n = 11), 0% and 0%, respectively. conclusions half of the isolates were mrsa, the majority of which harbored sccmec type iii. moreover, most of s. aureus isolates belonged to agri. the frequency of icaad, clfab, fib and eno genes were high in s. aureus species isolated from wounds. background staphylococcus aureus (s. aureus) isolates have emerged in healthcare and community, and cause a wide spectrum of clinical signs. on the other hand, methicilin-resistant s. aureus (mrsa) isolates can resist a wide range of antibiotics, which can make treatment of infections much more difficult. objectives the current study aimed to determine s. aureus characteristics isolated from wounds.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

ec adoption and critical success factors of ec in smes in iran

تجارت الکترونیکی شیوه اجرای مسئولیتها، تقابل با مشتریان و امور معمول عملیاتی در شرکتها را تغییر داده است. تجارت الکترونیکی در عمل تنها خرید و فروش کالاها را از طریق ابزار الکترونیک نمی باشد، بلکه تمام فعالیتهای لازم جهت انجام فرایند فروش را نیز در بر میگیرد. در اواخر دهه 1990 بسیاری از شرکتها فعالیتهای خود را به منظور دستیابی به مشتریان جدید و یا ارائه فرصتهای جدید به مشتریان موجود گسترش دادند...

The Frequency of Staphylococcus Aureus Isolated from Endocervix of Infertile Women in Northwest Iran

a:4:{s:9:"Objective";s:350:"Infertility is one of the major important social issues. Because of the asymptomatic cervical infection, it has caused the problem to remain silent in the majority of patients without diagnosis and treatment. The present study was intended to assess the frequency of Staphylococcus aureus isolated from infertile women's endocervix in northwest Iran.";s:19:"MaterialsAn...

متن کامل

High Frequency of biofilm related genes (icaD, fnbA, icaA and clfA) among Staphylococcus aureus isolated from bovine with subclinical mastitis

Background and aim: Regarding to the importance of Staphylococcus aureuscolonization in bovine mastitis and the role of biofilm in its pathogenesis, the aim of the present study were to assessment of biofilm production and identification of biofilm related genes among S. aureus isolated from bovine with subclinical mastitis. Materials and Methods: In this cross-sectional study, a total of 502 m...

متن کامل

Determination of hemolysine genes frequency in antibiotic-resistant Staphylococcus aureus isolated from wound and urine samples of patients

Background: Staphylococcus aureus is a pathogenic bacterium that can target host cell membranes with virulence factors such as toxins and peptides. This study was evaluated the frequency of alpha, beta, and delta hemolysine genes in antibiotic-resistant Staphylococcus aureus isolated from patients' urine and wound samples. Materials and methods: This cross-sectional study was performed on 100 a...

متن کامل

this study investigated the relationship between quality of work life and organization citizenship behavior from the viewpoint of teachers in high schools in tehran city.

چکیده هدف از پژوهش حاضر بررسی رابطه کیفیت زندگی کاری و رفتار شهروندی سازمانی در مدارس مقطع متوسطه پسرانه شهر تهران از دیدگاه دبیران بود. روش پژوهش حاضر توصیفی و از نوع همبستگی بود. از بین دبیران مدارس شهر تهران تعداد 380 نفر به روش نمونه گیری خوشه ای چند مرحله ای به عنوان نمونه پژوهش انتخاب شدند و با تکمیل پرسشنامه ی کیفیت زندگی کاری والتون(1975) و پرسشنامه ی رفتار شهروندی سازمانی پودساکو...

15 صفحه اول

The Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) Genes among Clinical Isolates of Staphylococcus aureus from Hospitalized Children

Background:Isolates of Staphylococcus aureus express a myriad of adhesive surface proteins that play important role in colonization of the bacteria on nasal and skin surfaces, beginning the process of pathogenesis. The aim of this study was to screen several of the Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) genes among the isolate of S. a...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
archives of clinical infectious diseases

جلد ۱۰، شماره ۴، صفحات ۰-۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023