اثر عصاره هیدروالکلی قارچ قیفی(Cantharellus cibarius) بر تغییرات بیان ژن‌های NT3 و NGF پس از کمپرسیون عصب سیاتیک در رت

Introduction: The expression of neurotrophic factors, which cause increasing of the survival and regeneration of neurons, is changed in response to nerve injury. Cantharellus cibarius has antioxidant and anti-inflammatory effects. So, the aim of present study was to determine the effects of hydro-alcoholic extract of Cantharellus cibarius on expression level of NT3 gene after sciatic nerve compression in rats. Methods: First the hydro-alcoholic extract of Cantharellus cibarius was prepared by the Soxhlet method. In this study, 36 Wistar male rats , 250-300 gr, were randomly divided into 3 groups consisted of 12 rats in each group. They were control, compression (1, 7, 14 and 28 days) and experimental (1, 7, 14 and 28 days) groups. Experimental groups were treated by 75 mg / kg of hydro-alcoholic extract of Cantharellus cibarius and to induce the stress in control group, saline serum was injected. In compression and experimental groups, the sciatic nerve of right leg was compressed for 60 seconds. The first injection of extract in the experimental group was performed intraperitoneally and immediately after the compression and the second one was injected 7 days later. Then, the sampling was performed of lumbar spinal cord on 1, 7, 14 and 28 days in compression and experimental groups and the total RNA was extracted from the spinal cord segments, cDNA was synthesized and after that the alteration of gene expression of NT3 and NGF samples was studied real time PCR method and Data were analyzed by Tukey test and SPSS 16 softwere. Results: The expression of NT3 and NGF showed significant increase in compression group compared to the control group, (p<0.05). Also, it is shown significant increase of expression of NT3 and NGF in the experimental group compared to the compression group, (p<0.05). Conclusion: According to these findings, hydro alcoholic extract of Cantharellus cibarius has an anti oxidant and anti-inflammatory effects that increase the regeneration process expression of NT3 and NGF in the experimental group was increase compared with the compression group.