ارتباط پلی مرفیسم IVS4 ژن PTEN با سرطان پستان زنان ایرانی

Abstract Background: Inactivation of tumor suppressor genes can cause irreversible uncontrolled growth. PTEN is a tumor suppressor gene and its polymorphism (IVS4) role in breast cancer was investigated in this study. Methods: Forty nine female subjects with confirmed breast cancer comprised the case group and 43 healthy women participated as a control group in this study. The PTEN polymorphism was genotyped by amplification of genomic DNA and then digestion by restriction of endonuclease and finally RFLP analysis. Results: The frequencies of mutant allele (M) were 0.37 and 0.27, respectively in the case and control groups. The genotype frequency distributions were in Hardy-Weinberg equilibrium in both groups. Conclusion: The mutant allele frequency in case group was 10% higher than control group it is possible that PTEN IVS4 polymorphism has a role in Breast Cancer. KEYWORDS: PTEN, IVS4, Breast Cancer, polymorphism.