Preparation and Characterisation of Immunoadsorbents for High Performance Liquid Affinity Chromatography (HPLAC)

The efficacy of activation methods and coupling were studied in the context of performance in batch and fixed bed binding experiments utilizing cell culture fluids or blood plasma as feedstock. Conclusions were drawn regarding selection of solid phase according to pore size, rigidity, pH stability, Chemistry of derivation and activation, and gross concentration of immobilized ligand required for optimal performance in analytical and preparative fractionation of complex feedstreams.