Studies on the Binding of DNA with the Inclusion of Brilliant Green inside the Cavity of γ-Cyclodextrin
Authors
Abstract:
The interaction of brilliant green with herring sperm DNA was investigated in detail by spectrometric methods in γ-cyclodextrin systems. On the condition of physiological pH, brilliant green prefers to form the 1:1 inclusion complex with γ-cyclodextrin.All the evidences indicated that the binding modes between γ-cyclodextrin-brilliant green and DNA were grooving binding and partial non-classical intercalative binding. The binding ratio of the inclusion complex with DNA is 6:1. The calculated thermodynamic parameters suggested that the binding of the inclusion complex to DNA was driven mainly by entropy.
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Journal title
volume 32 issue 2
pages 41- 47
publication date 2013-06-01
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