THE EFFECT OF INTENSE PERIODIC TRAINING AND CONSUMPTION OF BLACK GRAPE SEED EXTRACT ON PPARΑ AND PPARΓ GENE EXPRESSION IN PANCREATIC TISSUE OF MALE RATS WITH TYPE 2 DIABETES

Backgrond: Although some studies have studied the mechanism of action of beta cells in animal models and more or less in human populations, but so far the role of exercise therapy or exercise exercise HIIT along with black grape supplementation on the expression of involved genes Less has been studied in pancreatic beta cells, so the aim of the present study was to investigate the effect of eight weeks of intense intermittent exercise with black grape supplementation on the expression of PPARα and PPARγ gene in pancreatic tissue of male rats with type 2 diabetes. Methods: The study was conducted in the form of an experimental design. The subjects of this project were 40 8-month-old male rats with an average weight of 250 grams. After familiarizing the subjects with exercise and induction of diabetes by STZ, the subjects were randomly divided into 5 groups including exercise, supplement, exercise and supplement, diabetic control and basic control. After 8 weeks of training, 5 sessions per week for 8 weeks with 90% Vo2max activity and supplementation of black grape seed extract, PPARα and PPARγ genes were measured after RNA extraction from pancreas and cDNA synthesis. PPARα and PPARγ genes were measured by Time-Real PCR. Data were analyzed using independent t-test and two-factor analysis of variance at a significance level of 0.05 by SPSS software version 24. Results: The effect of exercise with consumption of black grape seed extract on the expression of PPARγ and PPARα genes in the pancreas of diabetic rats was not significant, but exercise alone significantly increased the expression of PPARα gene in the pancreatic tissue of diabetic specimens. Consumption of black grape seed extract alone significantly increased the expression of PPARγ and PPARα genes. Conclusion: It seems that regulating the expression of PPARα and PPARγ genes through exercise and consumption of black grape seed extract may lead to the improvement and maintenance of pancreatic beta cell function in diabetic rats.