The study of simultaneous resistance to heavy metals and antibiotics in resistant bacteria to silver and cadmium isolated from the wastewater

Authors

  • Ali Mohammad Ahadi Department of Genetics, College of Science, University of Shahrekord, Shahrekord, Iran.
  • Monir Doudi Dpatment of Microbiology , Falavarjan University, Islamic Azad University , Falavarjan, Isfahan, Iran
Abstract:

This laboratory study simultaneously was conducted to evaluate the resistance to heavy metals, silver, cadmium and a number of commonly used antibiotics. Sampling was done in 2014 of the two silver workshops in Isfahan, and the inlet of phase 2 of Shahin Shahr wastewater treatment plant, Isfahan. To isolate resistant bacteria to metal silver and cadmium and review multiple resistance to other metals in the medium PHG II agar with a concentration of 0.5 mM of metals and to determine the minimum inhibitory concentration (MIC) of growth, agar dilution method were used. To determine the resistance to several common antibiotics, disk diffusion techniques in agar with Kirby-Bauer method was used. In this study, molecular identification of bacteria was performed through gene sequencing 16S rDNA. New nucleotide sequences of the Cd1 and Cd2 isolates in GenBank’s database were deposited under accession numbers of KP753912 and KP753913. Most heavy metal-resistant isolated bacteria in this study had multiple resistance (MMR) compared to the Cadmium, Silver, Copper and Zinc metals and showed simultaneous resistance pattern to several heavy metals and antibiotics. Resistant isolates to cadmium compared to resistant isolates to silver had lower resistance and minimum inhibitory concentration (MIC), they also showed less resistance to a variety of antibiotics used in this study in a way that Cd2 isolate that was recognized as Dechloromonas hortensis strain MS2 was sensitive to all tested antibiotics. Since some of the isolates were highly sensitive to some of the antibiotics, the metal-resistant isolates and sensitive to antibiotic may be a proper candidates for biological removal of these metals from contaminated wastewater in the future.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

the study of simultaneous resistance to heavy metals and antibiotics in resistant bacteria to silver and cadmium isolated from the wastewater

this laboratory study simultaneously was conducted to evaluate the resistance to heavy metals, silver, cadmium and a number of commonly used antibiotics. sampling was done in 2014 of the two silver workshops in isfahan, and the inlet of phase 2 of shahin shahr wastewater treatment plant, isfahan. to isolate resistant bacteria to metal silver and cadmium and review multiple resistance to other m...

full text

the role of russia in transmission of energy from central asia and caucuses to european union

پس ازفروپاشی شوروی،رشد منابع نفت و گاز، آسیای میانه و قفقاز را در یک بازی ژئوپلتیکی انرژی قرار داده است. با در نظر گرفتن این منابع هیدروکربنی، این منطقه به یک میدانجنگ و رقابت تجاری برای بازی های ژئوپلتیکی قدرت های بزرگ جهانی تبدیل شده است. روسیه منطقه را به عنوان حیات خلوت خود تلقی نموده و علاقمند به حفظ حضورش می باشد تا همانند گذشته گاز طبیعی را به وسیله خط لوله مرکزی دریافت و به عنوان یک واس...

15 صفحه اول

from linguistics to literature: a linguistic approach to the study of linguistic deviations in the turkish divan of shahriar

chapter i provides an overview of structural linguistics and touches upon the saussurean dichotomies with the final goal of exploring their relevance to the stylistic studies of literature. to provide evidence for the singificance of the study, chapter ii deals with the controversial issue of linguistics and literature, and presents opposing views which, at the same time, have been central to t...

15 صفحه اول

contribution of setting to the study of characters and the main idea in e. brontes: wuthering heights and t. hardys: return of the native.

this thesis aims to adduce an unmitigated and comprehensive explication concerning the relationship of three significant elements of fiction: setting, chracter and theme. my research is basically placed on two outstanding novels of the 19th century: emily brontes wuthering heights and thomas hardys return of the native. my endeavour lies in studying the correlation among the three above-mention...

15 صفحه اول

a frame semantic approach to the study of translating cultural scripts in salingers franny and zooey

the frame semantic theory is a nascent approach in the area of translation studies which goes beyond the linguistic barriers and helps us to incorporate cognitive and cultural factors to the study of translation. based on rojos analytical model (2002b), which centered in the frames or knowledge structures activated in the text, the present research explores the various translation problems that...

15 صفحه اول

Antibiotics and heavy metals resistance patterns of Enterococcus faecalis and faecium bacteria isolated from the human and the livestock sources

Background: Enterococci have emerged as a major cause of nosocomial infections and within this group, Enterococcus faecalis and Enterococcus faecium cause the majority of human and livestock enterococcal infections. In this article, we tried to determine antibiotics and metals resistance patterns of E. faecalis and E. faecium strains. Methods: One hundred sixty different strains of E. faecalis...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 6  issue 1

pages  643- 651

publication date 2016-06-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023