Total Flavonoids Contents and Anti Bacterial Activity of the Extracts of two Labiateae Species: Nepeta menthoides and Thymus trautvetteri

Total flavonoids contents and antibacterial activity of the extracts from the aerial parts of two Labiateae species, Nepeta menthoides Boiss. & Buhse and Thymus trautvetteri Klokov & Desj.-Shost. were determined. Quantitative determination of flavonoid contents was calculated in terms of quercetin equivalent in various extracts (50% methanol, chloroform and distilled water) by AlCl3 colorimetric method. It was found that total flavonoids contents of N. menthoides extracts in different solvents were (2.308%, 0.884% and 0.710%) mg/g and (2.076%, 1.468% and 1.412%) mg/g for T. trautvetteri  respectively.Antibacterial activity of the extracts was also determined against 6 gr (+/-) bacteria by disc diffusion method. The results showed that 50% methanol extracts of two species displayed better inhibitor activity against the tested bacteria. The bacteria Stapylococcus epidermidis, Staphylococcus saprophyticus and Staphylococcus aureus  were sensitive against 50% methanol extract of N. menthoides even more than the standard antibiotics (10μg). Therefore a good correlation between total flavonoids contents and antibacterial activity of the studied extracts is demonstrated.