Seyed Abdulmajid Ayatollahi

Phytochemistry Research Center and Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

[ 1 ] - Design, Synthesis and Antitubercular Evaluation of Novel Series of Pyrazinecarboxamide Metal Complexes

The interest in the synthesis of metallic complexes of different drugs to make them moreefficient in biological environment of the human body is seen for the last few decades. Widerange of metal complexes are already used in clinical practice which encourages additionalresearch for innovating new metal based drugs, such as metal-mediated antibiotics, antiparasitic,antiviral, antibacterial, and ...

[ 2 ] - A New Taraxastane Triterpene from Euphorbia Denticulata with Cytotoxic Activity Against Prostate Cancer Cells

In this research, aerial parts of Euphorbia denticulata Lam were collected from Sanandaj city in Kurdistan province at the West of Iran. It was extracted by maceration using acetone as solvent. Isolation of compounds were carried out with repeated column chromatography using silica gel and normal preparative HPLC using YMC-Pack-Sil column and hexane: ethyl acetate as mobile phase. The structure...

[ 3 ] - Synthesis and evaluation of the antimicrobial activity of spiro-4H-pyran derivatives on some gram positive and gram negative bacteria

Infections are one of the most important causes of death, disability and inappropriate conditions for millions of people around the world. Therefore, the development in prognosis, prevention and treatment of infectious diseases made a considerable progress in designing and synthesis of new antimicrobial drugs. Nowadays, due to the increase in microbial resistance, discovery of new compounds wit...

[ 4 ] - Single Step Purification of Novel Thermostable and Chelator Resistant Amylase from Bacillus Licheniformis RM44 by Affinity Chromatography

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...

[ 5 ] - RP-HPLC method development and validation for determination of eptifibatide acetate in bulk drug substance and pharmaceutical dosage forms

A new, rapid, economical and isocratic reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of eptifibatide acetate, a small synthetic antiplatelet peptide, in bulk drug substance and pharmaceutical dosage forms. The developed method was validated as per of ICH guidelines. The chromatographic separation was achieved isocratically on C18 colum...

[ 6 ] - Chemical composition and biological activities of methanolic extract of Scrophularia oxysepala Boiss

Isolated five known iridoids glycosides (Scrophuloside A, Harpagoside B, 5-hydroxyloganin, 8-O-acetylharpagide and 6-O-methyl,1-glucopyranosyl catalpol ),one phenyl ethanoid glycoside (Verbascoside) and a phenyl ethanol amine (2-(4-Chlorobenzyl amino) ethanol ) compound from the methanolic extract of aerial parts of Scrophularia oxysepala using by high performance liquid chromatography based on...

[ 7 ] - Triterpene Constituents of Euphorbia Erythradenia Bioss. and their Anti-HIV Activity

Phytochemical investigation of the aerial parts of Euphorbia erythradenia Bioss. (Euphorbiaceae), one of Iranian endemic Euphorbias, with particular attention to triterpene constituents, using methanol solvent extraction was carried out. Five known triterpenes, including four cycloartanes and oleanolic acid, were isolated for the first time and identified using NMR and Mass techniques. Anti HIV...

[ 8 ] - Evaluation of anti-platelet aggregation effect of some Allium species

Epidemiologic studies show that the cardiovascular diseases are associated with multiple factors such as raised serum total cholesterol, increased LDL, increased platelet aggregation, hypertension and smoking. In-vitro studies have confirmed the ability of some plants of Allium species to reduce these parameters. Therefore, we evaluated anti-platelet aggregation effect of some Allium species (A...

[ 9 ] - Phytochemical investigations on chemical constituents of Achillea tenuifolia Lam.

Achillea tenuifolia Lam (Asteraceae) afforded a methanolic extract from which after fractionation in solvents with different polarities two known flavones 3’, 5- dihydroxy- 4’, 6, 7- trimethoxy flavone (eupatorine, compound 3), 5- hydroxy- 3’, 4’, 6, 7- tetramethoxyflavone (compound 4), besides stearic acid (compound 1 ), lupeol (compound 2), daucosterol (β- sitosterol 3-O- β- D- glucopyranosid...

[ 10 ] - Total Phenolic Content and Antibacterial Activity of Five Plants of Labiatae against Four Foodborne and Some Other Bacteria

The aim of this study was to evaluate the antibacterial effects of Thymus vulgaris, Thymus caramanicus, Zataria multiflora, Ziziphora clinopodioides and Ziziphora tenuior against four foodborne and four other bacteria including Staphylococcus aureus, Shigella dysenteriae, Salmonella typhimurium, Escherichia coli, Staphylococcus epidermidis, Bacillus subtilis, MRSA and Pseudomona aeruginosa and ...

[ 11 ] - Caffeine Treatment Prevented from Weight Regain after Calorie Shifting Diet Induced Weight Loss

Low calorie diets are always difficult for obese subjects to follow and lead to metabolic and behavioral adaptation. Therefore, we evaluated the effect of caffeine treatment with calorie shifting diet (CSD) on weight loss. Female subjects (n=98; BMI≥25) completed 4-weeks control diet, 6-weeks CSD (3 repeated phases; each 2-weeks) and 4-weeks follow-up diet, with or without caffeine treatment (5...

[ 12 ] - Cycloartane Triterpenoids from Euphorbia Macrostegia with Their Cytotoxicity against MDA-MB48 and MCF-7 Cancer Cell Lines

The dried plant was extracted with dichloromethane and after defatting with hexane, transferred repeatedly on silica columns using dichloromethane-hexane and ethyl acetate-hexane as mobile phases. Finally the fractions were purified by high performance liquid chromatography using a Pack-Sil column and hexane: Ethyl acetate as mobile phase. The structures of the isolated compounds included: cycl...

[ 13 ] - Triterpenes and steroids from Euphorbia denticulate Lam. with Anti-Herpes Symplex virus activity

In this research, dried acetone:chloroform extract of aerial parts of E. denticulata as one of the endemic plants to Iran, afforded a number of triterpenes and steroids including: betulin, 24-methylene-cycloart-3-ol, cycloart-23Z-ene-3β,25-diol, cycloart-23Z-ene-3β,25-diol, ergosta-8,24-dien-3-ol (obtusifoliol) and beta-sitosterol which were reported for the first time from this plant. The stru...

[ 14 ] - BCL-2 and Bax Expression in Skin Flaps Treated with Finasteride or Azelaic Acid

Despite all modern surgical techniques, skin flap that is considered as the main method in most reconstructive surgeries puts the skin tissue at danger of necrosis and apoptosis derived from ischemia. Therefore, finding a treatment for decreasing the apoptosis derived from flap ischemia will be useful in clinic. In present study, we evaluated the effect of azelaic acid 20% and finasteride on ex...

[ 15 ] - Flavonol Glycosides from Euphorbia microsciadia Bioss. with their Immunomodulatory Activities

Four known flavonoids: quercetin 3-O-β-D-rutinoside (Q3Rut), myricetin 3-O-β-D-galactopyranoside (M3Gal), quercetin 3-O-β-D-galactopyranoside (Q3Gal) and quercetin 3-O-β-D-glucopyranoside (Q3Glc), for the first time were isolated from aerial parts of Euphorbia microsciadia. The chemical structure of them was elucidated on the basis of 1 and 2 D-NMR spectra and different spectroscopic techniques...

[ 16 ] - Design, Synthesis and Antitubercular Evaluation of Novel Series of Pyrazinecarboxamide Metal Complexes

The interest in the synthesis of metallic complexes of different drugs to make them moreefficient in biological environment of the human body is seen for the last few decades. Widerange of metal complexes are already used in clinical practice which encourages additionalresearch for innovating new metal based drugs, such as metal-mediated antibiotics, antiparasitic,antiviral, antibacterial, and ...

[ 17 ] - A New Taraxastane Triterpene from Euphorbia Denticulata with Cytotoxic Activity Against Prostate Cancer Cells

In this research, aerial parts of Euphorbia denticulata Lam were collected from Sanandaj city in Kurdistan province at the West of Iran. It was extracted by maceration using acetone as solvent. Isolation of compounds were carried out with repeated column chromatography using silica gel and normal preparative HPLC using YMC-Pack-Sil column and hexane: ethyl acetate as mobile phase. The structure...

[ 18 ] - Synthesis and evaluation of the antimicrobial activity of spiro-4H-pyran derivatives on some gram positive and gram negative bacteria

Infections are one of the most important causes of death, disability and inappropriate conditions for millions of people around the world. Therefore, the development in prognosis, prevention and treatment of infectious diseases made a considerable progress in designing and synthesis of new antimicrobial drugs. Nowadays, due to the increase in microbial resistance, discovery of new compounds wit...

[ 19 ] - Single Step Purification of Novel Thermostable and Chelator Resistant Amylase from Bacillus Licheniformis RM44 by Affinity Chromatography

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...

[ 20 ] - RP-HPLC method development and validation for determination of eptifibatide acetate in bulk drug substance and pharmaceutical dosage forms

A new, rapid, economical and isocratic reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of eptifibatide acetate, a small synthetic antiplatelet peptide, in bulk drug substance and pharmaceutical dosage forms. The developed method was validated as per of ICH guidelines. The chromatographic separation was achieved isocratically on C18 colum...

[ 21 ] - Chemical composition and biological activities of methanolic extract of Scrophularia oxysepala Boiss

Isolated five known iridoids glycosides (Scrophuloside A, Harpagoside B, 5-hydroxyloganin, 8-O-acetylharpagide and 6-O-methyl,1-glucopyranosyl catalpol ),one phenyl ethanoid glycoside (Verbascoside) and a phenyl ethanol amine (2-(4-Chlorobenzyl amino) ethanol ) compound from the methanolic extract of aerial parts of Scrophularia oxysepala using by high performance liquid chromatography based on...

[ 22 ] - Triterpene Constituents of Euphorbia Erythradenia Bioss. and their Anti-HIV Activity

Phytochemical investigation of the aerial parts of Euphorbia erythradenia Bioss. (Euphorbiaceae), one of Iranian endemic Euphorbias, with particular attention to triterpene constituents, using methanol solvent extraction was carried out. Five known triterpenes, including four cycloartanes and oleanolic acid, were isolated for the first time and identified using NMR and Mass techniques. Anti HIV...

[ 23 ] - Evaluation of anti-platelet aggregation effect of some Allium species

Epidemiologic studies show that the cardiovascular diseases are associated with multiple factors such as raised serum total cholesterol, increased LDL, increased platelet aggregation, hypertension and smoking. In-vitro studies have confirmed the ability of some plants of Allium species to reduce these parameters. Therefore, we evaluated anti-platelet aggregation effect of some Allium species (A...

[ 24 ] - Phytochemical investigations on chemical constituents of Achillea tenuifolia Lam.

Achillea tenuifolia Lam (Asteraceae) afforded a methanolic extract from which after fractionation in solvents with different polarities two known flavones 3’, 5- dihydroxy- 4’, 6, 7- trimethoxy flavone (eupatorine, compound 3), 5- hydroxy- 3’, 4’, 6, 7- tetramethoxyflavone (compound 4), besides stearic acid (compound 1 ), lupeol (compound 2), daucosterol (β- sitosterol 3-O- β- D- glucopyranosid...

[ 25 ] - Total Phenolic Content and Antibacterial Activity of Five Plants of Labiatae against Four Foodborne and Some Other Bacteria

The aim of this study was to evaluate the antibacterial effects of Thymus vulgaris, Thymus caramanicus, Zataria multiflora, Ziziphora clinopodioides and Ziziphora tenuior against four foodborne and four other bacteria including Staphylococcus aureus, Shigella dysenteriae, Salmonella typhimurium, Escherichia coli, Staphylococcus epidermidis, Bacillus subtilis, MRSA and Pseudomona aeruginosa and ...

[ 26 ] - Caffeine Treatment Prevented from Weight Regain after Calorie Shifting Diet Induced Weight Loss

Low calorie diets are always difficult for obese subjects to follow and lead to metabolic and behavioral adaptation. Therefore, we evaluated the effect of caffeine treatment with calorie shifting diet (CSD) on weight loss. Female subjects (n=98; BMI≥25) completed 4-weeks control diet, 6-weeks CSD (3 repeated phases; each 2-weeks) and 4-weeks follow-up diet, with or without caffeine treatment (5...

[ 27 ] - Cycloartane Triterpenoids from Euphorbia Macrostegia with Their Cytotoxicity against MDA-MB48 and MCF-7 Cancer Cell Lines

The dried plant was extracted with dichloromethane and after defatting with hexane, transferred repeatedly on silica columns using dichloromethane-hexane and ethyl acetate-hexane as mobile phases. Finally the fractions were purified by high performance liquid chromatography using a Pack-Sil column and hexane: Ethyl acetate as mobile phase. The structures of the isolated compounds included: cycl...

[ 28 ] - Triterpenes and steroids from Euphorbia denticulate Lam. with Anti-Herpes Symplex virus activity

In this research, dried acetone:chloroform extract of aerial parts of E. denticulata as one of the endemic plants to Iran, afforded a number of triterpenes and steroids including: betulin, 24-methylene-cycloart-3-ol, cycloart-23Z-ene-3β,25-diol, cycloart-23Z-ene-3β,25-diol, ergosta-8,24-dien-3-ol (obtusifoliol) and beta-sitosterol which were reported for the first time from this plant. The stru...

[ 29 ] - BCL-2 and Bax Expression in Skin Flaps Treated with Finasteride or Azelaic Acid

Despite all modern surgical techniques, skin flap that is considered as the main method in most reconstructive surgeries puts the skin tissue at danger of necrosis and apoptosis derived from ischemia. Therefore, finding a treatment for decreasing the apoptosis derived from flap ischemia will be useful in clinic. In present study, we evaluated the effect of azelaic acid 20% and finasteride on ex...

[ 30 ] - Flavonol Glycosides from Euphorbia microsciadia Bioss. with their Immunomodulatory Activities

Four known flavonoids: quercetin 3-O-β-D-rutinoside (Q3Rut), myricetin 3-O-β-D-galactopyranoside (M3Gal), quercetin 3-O-β-D-galactopyranoside (Q3Gal) and quercetin 3-O-β-D-glucopyranoside (Q3Glc), for the first time were isolated from aerial parts of Euphorbia microsciadia. The chemical structure of them was elucidated on the basis of 1 and 2 D-NMR spectra and different spectroscopic techniques...

[ 31 ] - Development and Validation of a GC/MS Method for Simultaneous Determination of 7 Monoterpens in Two Commercial Pharmaceutical Dosage Forms

Terpenes are active constituents of many pharmaceutical dosage forms with naturalproducts origin. One of the challenges in developing dosage forms with herbal originis their standardization as pharmaceutical products. GC-Mass is the most decisive andreliable technique to fulfill the requirements in this regard. In the present study, a reliable,rapid, and accurate method was developed for determ...

[ 32 ] - Development and Validation of a GC/MS Method for Simultaneous Determination of 7 Monoterpens in Two Commercial Pharmaceutical Dosage Forms

Terpenes are active constituents of many pharmaceutical dosage forms with naturalproducts origin. One of the challenges in developing dosage forms with herbal originis their standardization as pharmaceutical products. GC-Mass is the most decisive andreliable technique to fulfill the requirements in this regard. In the present study, a reliable,rapid, and accurate method was developed for determ...

[ 33 ] - Effects of saffron oral gavage on protection of skin flaps against tissue necrosis and oxidative stress in rats

Introduction: Today, skin flaps are used in many reconstructive surgeries and healing of skin lesions as one of the main methods of treatment. Despite improvements in the fields related to enhancement of skin flap survival, flaps viability and their efficacy are still threatened with issues such as ischemia-reperfusion, oxidative stress, tissue necrosis, and even apoptosis in the affected tissu...

[ 34 ] - Comparison Cytotoxic Effects of Mangifera Indica L. and Juglans Regia Aqueous Extract on Human Chronic Lymphocytic Leukemia

Natural products isolated from plant sources are well known for their pharmacological potential in diversity of disease treatments such as inflammatory or cancer conditions. Mango (Mangifera indica L.) and Juglans regia are thought to be rich of functional phytochemicals. To elucidate the anticancer activity of Juglans regia (JR) and Mangifera indica L (MI) aqueous extract were investigated on ...

[ 35 ] - Comparison Cytotoxic Effects of Mangifera Indica L. and Juglans Regia Aqueous Extract on Human Chronic Lymphocytic Leukemia

Natural products isolated from plant sources are well known for their pharmacological potential in diversity of disease treatments such as inflammatory or cancer conditions. Mango (Mangifera indica L.) and Juglans regia are thought to be rich of functional phytochemicals. To elucidate the anticancer activity of Juglans regia (JR) and Mangifera indica L (MI) aqueous extract were investigated on ...

[ 36 ] - Metal (II) Complexes of Fluconazole: Thermal, XRD and Cytotoxicity Studies

We report thermal, X-ray diffraction (XRD) and cytotoxicity studies of complexes of fluconazole (FCZ) with Cu (II), Fe(II), Cd(II), Co(II), Ni(II), and Mn(II). From XRD measurements, FCZ and its metal complexes were identified as polycrystalline. Marked differences in the X-ray patterns of drug and its metal complexes revealed that the complexes are indeed different compounds and not just the m...

[ 37 ] - Metal (II) Complexes of Fluconazole: Thermal, XRD and Cytotoxicity Studies

We report thermal, X-ray diffraction (XRD) and cytotoxicity studies of complexes of fluconazole (FCZ) with Cu (II), Fe(II), Cd(II), Co(II), Ni(II), and Mn(II). From XRD measurements, FCZ and its metal complexes were identified as polycrystalline. Marked differences in the X-ray patterns of drug and its metal complexes revealed that the complexes are indeed different compounds and not just the m...

Co-Authors

Farzad Kobarfard 10  

Sayyed Mustafa Ghannadian 8  

Mansoor Ahmed 6  

Mohsin Ali 6  

Mustafa Kamal 4  

Mahmoud Aghaei 4  

Mohammad reza Rahiminejad 4  

Sara Shamsabadipour 4  

Hamidreza Pazoki-Toroudi 4  

Sayed Hossein Davoodi 4  

Varshoei, Behnam 3  

Majid Mumtaz 2  

Saleem Hafiz 2  

Hojjatollah Saeedi 2  

Seyed Mohammad Zarei 2  

Alieh Bazi 2  

Hadi Dolati 2  

Laleh Rafighdoost 2  

Marzieh Amirmostofian 2  

Pardis Nazari 2  

SeyedehMahbobeh Mahdavi 2  

Ayesha Siddiqui 2  

Hossein Vahidi 2  

Maryam Bavand Savadkouhi 2  

Shirin Hooshfar 2  

Abbas Delazar 2  

Ardalan Pasdaran Lashgari 2  

Arsalan Pasdaran 2  

Arash Memarnejadian 2  

Mohammad Heydarian Moghadam 2  

Seyed Mohammad Zarei 2  

Salimeh Amidi 2  

Zahra Lorigooini 2  

Shirin Moradkhani 2  

Arash Mahboubi 2  

Mohammad Babaeian 2  

Mohammad Kamalinejad 2  

Anoushiravan Mohseni-Bandpei 2  

gholamali javedan 2  

Kamran Dowlatshahi 2  

marjan ajami 2  

Hojjatollah Saeidi 2  

Mustafa Ghannadian 2  

Somayeh Baniadam 2  

Marzieh Mohammadi-Kamalabadi 2  

Esmaeil Habibi 2  

Mansour Nassiri-Kashani 2  

Marjan Ajami 2  

Mehdi Rashighi Firoozabadi 2  

Yasin Asadi 2  

Jean Jules Kezetas Bankeu 2  

Suleiman Afsharypour 2  

Sumaira Hareem 2  

Ali Goodarzi 2  

Athar Ata 2  

Marjan Esfahanizadeh 2  

Mitra Bayat 2  

Ahmad Salimi 2  

Jalal Pourahmad 2  

Jalil Rahmati 2  

Dong-Hua Yang 2  

Konatsu Kojima 2  

Richard Peng 2  

Syed Imran Ali 2  

Syed Moazzam Haider 2  

Zhe-Sheng Chen 2  

Zi-Ning Lei 2