Kazem Mashayekhi
ImmunoBiochemistry lab, Immunology Research Center, Mashhad University of medical Sciences, Mashhad, Iran
[ 1 ] - Optimization of Cloning Conditions for High-Level Production of Recombinant Mouse Interleukin-2 in Escherichia coli
Backgrounds Many proteins have been expressed so far in bacterial host. Due to its simple culture conditions, having a short life cycle, and easily genetic manipulation, E.coli have been regarded as a preferable host to produce recombinant proteins, but protein cloning in bacterial host have many challenges. Therefore, we aimed to review some of these problems by an experience from mice IL-2 reco…
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