بررسی اثرات ضد توموری و ضد التهابی گیاه سیاه دانه روی رده های توموری 4T1 و CT26

Introduction: Globally, cancer is the second leading cause of death. Cancer-related inflammation plays an important role in malignant progression of several cancer types. Current therapeutic approaches are not completely effective for cancer therapy. In addition, they are associated with high side effects. Since medical herbs have fewer side effects than chemical drugs. Lots of attention has been paid to the use of herbal extracts in cancer treatment. Therefore, the present study aimed to investigate the effects of anti-tumor and anti-inflammatory of Nigella sativa in in vitro conditions. Methods and Materials: In this study, 4T1 and CT26 cell lines were treated with different concentrations of hydro alcoholic extracts of Nigella sativa (50, 250, 500, 1000 &mu;g/mL). After 48hours, the effects of the extract on tumor growth and COX-2 gene expression were analyzed by XTT and Real time PCR, respectively. Results: The results revealed that the 4T1 and CT26 cell lines growth significantly decreased in a dose dependent manner comparing to the control group (P<0.05). Moreover , Real time PCR results indicated that the expression of COX-2 significantly decreased in a dose dependent manner even at the low concentration of extract comparing to the control group (P<0.05). Discussion and Conclusion: Based on the results, Nigella sativa extract not only suppressed growth of cancer cell lines, but also significantly reduced cancer-related inflammation. Therefore, it may be utilized as a new therapy against several types of cancer.