جداسازی زنجیره‌های گلوبین به روش کروماتوگرافی تعویض یونی در تشخیص هموگلوبینوپاتی‌ها

Background & Aim: Since the mutant Hbs do not have any obvious electrical charge, globin chain separation is helpful for the diagnosis ofunknown Hbs. Therefore, the present study was carried out to detect alpha or beta chain variants by cation exchangechromatography.There are several point mutations in hemoglobin(Hb) genes which can cause hemoglobinopathy.Material and Method: count(CBC), HbA2 and HbF percentages were determined by routine methods, and cellulose acetate and citrate agarelectrophoresis were performed on all the samples. For HbS confirmation, solubility test was performed and globin chainswere separated by carboxymethyl cellulose(CMC) chromatography in the presence of 8 M urea. SPSS version 14 was usedfor statistical analysis.In this descriptive study, 20 samples having abnormal Hb were selected. Complete blood cellResults: agar electrophoresis. CMC chromatography revealed that three patients had abnormal beta chains, 15 had abnormal alphachains, and 2 remaining samples had abnormal alpha and beta chains.According to the obtained results, all of the samples had an abnormal band on cellulose acetate and citrateConclusion: helpful guideline for the selection of an appropriate gene for DNA sequencing. Moreover, this method is useful to screen thecases for hemoglobinopathies and beta thalassemia coexistance, which can sometimes lead to severe clinicalmanifestations.Mutant alpha and beta chains can be detected by CMC chromatography and globin chain separation is a