طراحی و ساخت نانولیپوزوم آهسته رهش حاوی داروی گیاهی ضدسرطان سیلیبینین (نانوفایتوزوم)

Introduction: Nano-liposomes are Nano particulate vesicles with lipoid membrane which are under extensive investigation as drug carriers for improving the delivery of therapeutic agents. This study intended to enhance efficacy of Silibinin herbal drug in Nano liposome system (Nano phytosome) via encapsulation for delivery to liver cancer cells. Silibinin is one of the anti-cancer drugs, which its antitumor efficacy is primarily attributed to decreasing N-nitrosodiethylamine in hepatocit carcinoma cells. Nano Liposome encapsulation of Silibinin can dramatically improve its biological activity and increase stability of Silibinin in blood. Methods: Small uni-lamellar (SUV) vesicles entrapping Silibinin were prepared using DiPalmitoyl PhosphatidylCholine (DPPC), cholesterol: DSPE-MPEG2000 at 7:4:0.36 molar ratio , the fluorescent label (DIL) incorporated in the lipid bilayer at 0.09 mol % as lipophilic phase and buffer of HEPES as hydrophilic phase. Moreover, Nano Liposome size, Zeta-potential, encapsulation efficiency and release of drug were determined after Nano Liposome production . Results: The study results demonstrated that mean nano-liposome diameter was 46.3 nm. The size and structure of Nano-liposomes were analyzed by SEM and TEM images. The zeta potential of the encapsulated Nano-liposomes was shown -23.25. The encapsulation efficiency for Silibinin was about 24.37%. Conclusion: In this study, silibinin drug encapsulated nano-liposome controlled release system to improve the solubility and bioavailability of silibinin for delivery to liver cancer cells.