Evaluation of cultivated and wild barley cultivars affinities using micro and macro-morphological traits of grain, pollen, and stomata. Sayyedh Masomeh Hosseini 1*, Mahlagh Ghorbanli 2 and Hossein Sabouri 3

In an ongoing research, 24 cultivated and wild cultivars of barley were evaluated for morphological characteristics of grain, pollen, and stomata. Traits of interest included length, width, and area. Results of variance analysis showed that all samples were differed in traits of stomata, grain, and pollen at probability levels of 1 and 5%, suggesting remarkable genetic variation among studied samples. According to cluster analysis, morphological traits of pollen and stomata fell into three cluster and those for grain were categorized in two clusters. Genotypes for arid area like Izeh, Maravetapeh, Kavir, and Nimrooz showed higher levels of stomata densities and area compared to those for cold area like Bahman and A1C84-14. Here, affinity rate of 24 barley cultivars were evaluated using micro and macro-morphological traits as those rare-studied ones.