Optimized Root Production During Micropropagation of New Iranian Apple Hybrid Rootstock (AZ X M9): Effects of Fe-EDDHA and Thiamine

نویسندگان

  • A. Yadollahi Department of Horticultural Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
  • D. Atashkar Department of Horticultural Sciences, Seed and Plant Improvement Institute, Karaj, Iran
  • M. Eftekhari Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Islamic Azad University, Science and Research Branch, Tehran, Iran
  • M. Haghgou Tabalvandani Department of Plant Breeding, Faculty of Agriculture and Natural Resources, Islamic Azad University, Science and Research Branch, Tehran, Iran
چکیده مقاله:

Objective: In vitro propagation of AZ x M9 new apple rootstock resulted of breeding program of vegetative apple rootstock in Iran was investigated. Methods: Nodal explants were inoculated in Murashige and SKoog (MS) medium supplemented with various concentrations of 6-benzylaminon purine (BAP) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (NAA) (0.1 mg/l). We examined the effect of different concentrations of iron sequestrene (Fe-EDDHA) and thiamin vitamin in ½ MS and Linsmaier and Skoog (LS) media supplemented with indol-3-butric acid (IBA) (1 mg/l) on rooting of attained plantlets. Results: According to our results, 0.4 BAP + 0.1 NAA treatment in MS medium was the best component which resulted in the highest proliferation rate (6.73). As well, we estimated the highest root number in ½ MS + 0.15 g/l Fe-EDDHA + 2.4 g/l thiamin.

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optimized root production during micropropagation of new iranian apple hybrid rootstock (az x m9): effects of fe-eddha and thiamine

in vitro propagation of az x m9 new apple rootstock resulted of breeding program of vegetative apple rootstock in iran was investigated. nodal explants were inoculated in murashige and skoog (ms) medium supplemented with various concentrations of 6-benzylaminon purine (bap) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (naa) (0.1 mg/l). we examined the effect of different concen...

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عنوان ژورنال

دوره 2  شماره 10

صفحات  2659- 2662

تاریخ انتشار 2014-10-01

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