P-101: Study of The Effect of Alkaline Medium on Separation of Spermatozoa Containing X and Y Chromosomes in order to Sex Selection

Background: Sex Selection in livestock happens in order to increase productivity and it also occurs in human society to avoid genetic disease related to gender and culture, social and religious trends. By separating spermatozoa carrying X and Y chromosomes from each other, we could achieve a desire gender by using assisted productive techniques. In this study the effect of alkaline environment were examined on the speed of spermatozoa carrying X and Y chromosomes Materials and Methods: A number of 60 mice (NMRI) were divided in to a control group and three experimental groups. Female mice stimulated by injecting PMSG matter to supper ovulation. Oocyte retrieved under sterile condition from oviduct. Spermatozoa provided from cauda epididymis and vas deferan and entered to media by pH(7.5, 7.6, 7.7). The faster spermatozoa were used for IVF. The embryo in control and experimental groups were transferred to foster mother(Pseudo pregnant) and the female and male infants were counted after birth. Analysis of data has been done with the help of spss, Anova software. Results: The proportion of male and female in control group(pH=7.3), experimental group 1 (pH=7.5) and experimental group2 (pH=7.6) and experimental group3 (pH=7.7) was (47.98),(64.75), (65.41), (67.31) (Mean±SD) respectively. The comparison of proportion of male to female infant between control group and experimental groups showed significant differences (p <0.05). But there was not significant difference among experimental groups. Conclusion: With respect of results of this study, it is appear that by increasing the pH of media up to 7.7, the speed of spermatozoa carrying Y chromosom will increase. Therfore, by increase of pH of spermatozoa media, the chance of male infant birth will increase.