P-109: Effect of Chaste Tree Essential Oil on Sperm In Vitro Fertilization (IVF) Index in Mice
Background: Chaste tree is a phytoestrogenic herb and has a long tradition as a herbal remedy and was used in ancient times as an aphrodisiac. This study was conducted to evaluate in vitro fertilization ability and cleavage rate of sperm in chaste tree essential oil treated male mice. Materials and Methods: In this study, 10 adult male mice were divided in to two groups: group 1 as control and group 2 which received 75 mg/kg essential oil via gastric gavage for seven consecutive days. Animals were housted at a controlled condition in accordance with humane care and animal welfare. After this time, animals sacrificed by cervical dislocation. The epididymes dissected out and spermatozoa expressed out by cutting the distal end of the cauda epididymidal tubule. For in vitro fertilization (IVF), superovulation induced by hMG and hCG (10 IU) in normal female and oocytes recovered from ampulla. Results: Results revelved that fertilization ability and IVF index decreased significantly in treated males (p<0.05). However pronucleus formation was not different between groups but results showed that cleavage rate has a reduction in the sperm of mices received chaste tree essential oil. Conclusion: It can be concluded that chaste tree essential oil can reduce fertilization capacity of sperm.
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Background Oxidative stress induced by cyclophosphamide (CP), as a chemotherapeutic agent, causes vulnerability of sperm and declining the fertility capacity. Then in this study the effect of crocin (saffron extract) on amelioration of these side effects was investigated. MaterialsAndMethods In this study 24 mice were divided in three groups (n=8). Control group received normal saline (0.2 ml/d...متن کامل
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
Background: One of the major factors affecting on male infertility is oxidative stress (OS). Two main sources of ROS production are leukocytes and spermatozoa. The ROS such as tertiary-butyl hydroperoxide (TBHP) may damage and fragmentate to sperm DNA, which can lead to complications in the offspring. In this study, we will investigate the effect of od on DNA fragmentation index (DFI) in sperm ...متن کامل
Ameliorative Effect of Crocin on Sperm Parameters and In Vitro Fertilization in Mice under Oxidative Stress Induced by Paraquat
Objective Background and Objectives: Paraquat (PQ) is a herbicide and is genotoxic and cytotoxic poison for male germ cells. Therefore, in this study, the protective role of Crocin (Cr) against the destructive effects of Paraquat on sperm quality and in-vitro fertilization results Paraquat was investigated. MaterialsAndMethods Materials and Methods: Twenty-eight male mice (20-25g) were used in ...متن کامل
Background: Plant materials continue to play an important role in the maintenance of human health since antiquity. Phytoestrogens are naturally occurring plant substances that can either mimic or antagonize the action of endogenous estrogens. This study was conducted to investigate the effects of vitex agnus as a phytoestrogenic plants, essential oil on structure and morphology of seminiferous ...متن کامل
دوره 6 شماره 2
تاریخ انتشار 2012-09-01
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