P-118: Effect of Vitrification on Developmental Competence of Parthenogenetic Activation in In Vitro Matured Ovine Oocytes
Background: Cryopreservation of in vitro matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies. On the other hand, the cryopreservation of oocytes is an open problem as a result of their structural sensitivity to the freezing process. The purpose of this study was to evaluate the effect of vitrification on in vitro development of vitrified in vitro matured ovine oocytes after chemical activation. Materials and Methods: Immature oocytes were collected from abattoir-derived ovaries, matured in vitro. Then, in vitro matured oocytes divided into two groups: 1. vitrified in cryotop (VTR); 2. without treatment as a control (CTR). 407 matured ovine oocytes were cryopreserved by vitrification. oocytes were exposed to 7.5% EG + 7.5% DMSO for 3 minutes and then 15% EG + 15% DMSO + 0.5 M sucrose for 25 seconds, loaded in cryotops and immersed into liquid nitrogen. After warming, oocytes were cultured in vitro for 30 minutes and then parthenogenically activated using ionomycin for 1 minute and subsequently incubated in 6-dimethylaminopurine (6-DMAP) for 2 hours. Results: When vitrified-warmed oocytes were activated, blastocyst rates in VTR (10.21%) group was significantly lower (p< 0.05) than in CTR (39.50%). Conclusion: Vitrification procedures effect on the structural components and biochemical and molecular events such as spontaneous parthenogenetic activation that could be a reflection of injuries to cytoplasmatic biochemical components leading to abnormalities in the cell cycle control, degeneration and low developmental competence of vitrified MII ovine oocytes.
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
A comparative study of parthenogenetic activation and in vitro fertilization of in vitro matured caprine oocytes
The aim of the study was to compare the parthenogenetic activation and in vitro fertilization (IVF) of in vitro matured caprine oocytes. A total of 881 cumulus-oocyte complexes (COC’s) were collected from 243 ovaries. Oocytes were matured in TCM-199 medium containing eCG (20 IU/ml), hCG (20 IUμg/ml), oestradiol-17β (1 μg/ml), BSA embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...متن کامل
P-92: Specific Activation Requirements of In Vitro Matured Sheep Oocytes following Vitrification-Warming
Background: Oocyte vitrification and assisted oocyte activation have increasingly important implications in assisted reproductive technology. However, an important area of concern with matured oocyte cryobiology is that elements of oocytes intimately involved in metaphase-II arrest may be modified by cryopreservation. In a comparison between different cellular characteristics of unvitrified, vi...متن کامل
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
P-118: Developmental Competence of Ovine Embryos Derived from Frozen Oocytes Co-Cultured with Embryos Derived from Fresh Oocytes
Background: Currently, a limited number of studies have been done on the vitrification of small ruminant oocytes, especially in sheep, where poor developmental rates are obtained following immature and mature oocyte vitrification. Poor ovine oocyte cryopreservation could attribute to the damage to enzymes which has role in metabolic pathways. Therefore, production of cytokines and growth factor...متن کامل
Background: Despite great advances achieved in the field of in vitro oocyte maturation (IVM), almost all IVM media are supplemented with undefined component which not only pose the risk of pathogen transmission but also hinders our knowledge of molecular mechanisms of maturation. One of these components is serum that containing various concentration of unknown molecules affecting cellular proce...متن کامل
دوره 6 شماره 2
تاریخ انتشار 2012-09-01
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