P-17: Expression of Cell Surface Toll-Like Receptors in the Human Male Reproductive Tract
Background: Male infertility refers to the inability of a male to achieve a pregnancy in a fertile female. The root of many causes of infertility is miscommunication between immune and reproductive system. Male reproductive system is very sensitive and vulnerable, infections can hinder maturation and movement of spermatozoa lead to impaired fertility.All species need an immediate reply to the microbial pathogens that is part of an effective immune response. This reply is known as the innate immune response. The innate immune system uses a series of pattern recognition receptors to detect the presence of pathogens. A key component of such phenomena receptors are Toll-like receptors (TLRs). TLRs are a family of receptors that recognize different pathogen-associated molecular patterns (PAMPs) present in pathogens. To date, 10 functional members of TLRs have been discovered in human. Among them TLR1, TLR2, TLR4, TLR5, TLR6 and TLR10 are expressed on the cell surface while others are expressed in cytoplasmic organelles, mainly the endosomes. Cell surface TLRs mostly identified bacterial and fungal infections. TLR2 and its associated receptors TLR1, TLR6 and TLR10 are mainly involved in the detection of molecules from Gram-positive bacteria. However, TLR4 and TLR5 recognize mainly gram-negative bacterials. TLR4 is a receptor for bacterial LPS and chlamydial heat shock proteins. While, TLR5 can recognize flagellin of bacterial and fungi. Considering to the important roles of innate immunity in reproductive health, the objective in this study is detecting cell surface TLRs expression in the human male reproductive tract and spermatozoa. Materials and Methods: Tissues were obtained from men who underwent TESE, Vasectomy, Prostatectomy for benign reasons and Prepuce surgery. All men taking part in this study had not history of infection and congenital disorder. RT-PCR was used to show the existence of cell surface TLR genes in the male reproductive tract, Q-PCR analysis used to investigate the relative expression of TLR2 and TLR4 genes and Immunoblot analysis was used for detect TLR2 and TLR4 on spermatozoa. Results: All cell surface TLR mRNAs were expressed in all regions of the human male reproductive tract. Q-PCR has shown relative TLR2 expression in TESE (-) patient is lower than TESE (+) patient while there was no difference between TLR4 expression in TESE (+) compared to TESE (-). Existence of TLR2 and 4 in spermatozoa has been shown by using immunoblot. Conclusion: Because, cell surface TLRs mostly identified bacterial and fungal infections, the expression of cell surface TLR mRNAs in the human male reproductive tract and TLR2 and TLR4 expression on spermatozoa provides strong evidence that they play important roles in innate immunity of the male reproductive tract. Also TLR2 expression in testis is affected by the presence of spermatozoa while spermatogenesis have no effect on TLR4 expression in testis.
Background: In the past decade, childlessness has become a most important problem in the world. At the same time evidence confirms a link between sexually transmitted diseases (STDs) and infertility problem. Some of viral STDs are: HIV, HPV, HSVand so on. The innate immune system is essential for the initial detection of invading viruses and subsequent activation of adaptive immunity. also, The...متن کامل
Background: Toll like receptors (TLRs) are a major family of innate immune systems which recognize specific pathogen associated molecular patterns (PAMPS)in bacterial, fungi, virus and parasites. Human TLRs comprise a large family of 10 different type proteins that are expressed on various immune cells. Among these receptors, TLR1, 2, 4, 5, 6 and 10 were expressed on the cell surface. TLR2 form...متن کامل
Background: Endometriosis is a complex disease that profoundly affects the quality of life in many women. This disease affects roughly one in ten women of reproductive age. Endometriosis induces a variable amount of inflammatory reaction in pelvic environment. An active immune system needs to recognize these inflammatory agents. Rapid innate immune system defenses against infections involve the...متن کامل
Background: Endometriosis is the growth of endometrial cells outside the uterine cavity. It has been suggested that immune system plays important roles in both initiation and progression of the disease. Several studies have been shown that women with endometriosis diverge in their expression of different genes including heat-shock proteins, fibronectin, and neutrophil elastase, which might be i...متن کامل
Background: Toll-like receptors (TLRs) constitute a major part of innate immunity, which can distinguish pathogen associate molecular pattern. Sertoli cells create a special immunological niche that protects somniferous tubules from auto antigens and pathogens. These cells are the only somatic cells in somniferous that protect testis cells against pathogens. The purpose of this study was to eva...متن کامل
Breast cancer is highly prevalent worldwide. Treatment of this disease is a major global public health problem; therefore, numerous studies have explored new molecular profile helping for breast cancer therapy. Different risk factors are attributed to breast cancer; however, chronic inflammation and immune regulation are highly essential. Various molecules impact this context, including toll-li...متن کامل
دوره 5 شماره Supplement Issue
تاریخ انتشار 2011-09-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.