P-223: Analysis of Synaptonemal Complex Gene Disorders Involving in Recurrent Spontaneous Abortion

Background: Spontaneous abortion (SAb) is the most common complication of early pregnancy. Numerous risk factors are associated with an increased risk of pregnancy loss such as: Maternally age, previous spontaneous abortion, prolonged ovulation to implantation, Gravidity, Interval Prolonged time to pregnancy, Balanced chromosomal translocations and Genetic disorders. The aim of this study was to determine the frequency of balanced chromosomal translocation in couples with a history of recurrent spontaneous and possibly genetic disorder which may be involved in synaptonemal complex during the posses of meiosis. Materials and Methods: Sixty eight couples with the history of spontaneous abortion were selected and introduced into this survey during 2007-2012 (department of Medical Genetics department of Mashhad University). Giemsa banding technique was used to searching for chromosomal balanced translocation. However it should be mentioned that, this techniques will not be able to detect balanced chromosomal micro rearrangements which are behind the 4 mega base pair. PCR technique was used in order to diagnose of some synaptonemal complex involving genes disorders in spontaneous abortion. Results: Results of our research are gathering and doing with PCR for diagnosis in synaptonemal complex gene disorders as soon as possibility. Conclusion: It would be reasonable to suggest that single gene determinants may play an important role in such complication pregnancy than synaptonemal complex in chromosomal disorders.