P-54: A Study on The Presence of CD52and CD9 on Sperm in Infertile Couples Who Are Candidate for Intra Utreine Insemination

Background: Fertilization and fecundation obviously are the phenomenon in which several factors are involved. Sperm membranous proteins are a series of these important elements. CD9, an inner acrosomal membrane protein, is one of the tetraspanin members that involved in sperm-egg fusion. CD52, is a GPI anchor protein which is expressed in the epididymal cells and during passage of sperms through epididimis added to the sperm surface. The aim of this study was to obtain any association between the percentage of CD52 positive sperm with sperm parameters and also evaluation of correlation between the percentage of CD9 positive sperm with outcome of IUI. Materials and Methods: A total of 120 semen samples were collected from infertile couples who were undergoing IUI treatment. Samples evaluated according to WHO 2010 guidelines. Concentration and motility parameters were measured by means of computer assisted semen analysis (CASA). Parameters recorded were motile cells, progressive motility, average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL) and linearity (LIN). Sperm washed and immunostained using antibody. Results: This study indicates that CD9 is detected in only 19% of mature sperm. Regarding the results, the area under the ROC curve is equal to 0.446 and CD9 is not a good predictor variable for fertility status (p=0.482). On sperm kinematic parameters there is a significant positive correlation between the percentage of CD52 expression with avg.VCL(r=0.282,p=0.013), avg.VSL (r=0.336,p=0.003) and avg.VAP (r=0.409,p=0.00) and normal morphology (r=0.866,p=0.00). No significant correlation was found between the percentage of CD52 with avg.LIN (r=0.020,p=0.860) and sperm count (r=0.125,p=0.278). Conclusion: The results indicated the positive correlation between the percentage of CD52 positive sperm with normal morphology and sperm kinematics. The results did not illustrated any relation between the proportion of CD9 expression and pregnancy in IUI patients.