P-79: The Effect of Alkaline Environments Made with Calcium Carbonate in Order to Migration Spermatozoa for Sex Selection

Background: Due to different such as some sexrelated illnesses, spouses have been encourage to designate special treatments for assessing sexuality. Moreover, Utilizing from identifying sexulityb has been an important aim in industry in order to product humans needs and develop cattle. In this research, the effect of alkaline environments were examined migration spermatozoa for sex selection. Materials and Methods: A number of 40 mice (NMRI) were divided in to a control group and three experimental group. Ovulation of mice was stimulated by using PMSG and HCG hormones. Female mice were killed by cervical dislocation and dissect. Oocytes were collected stril condition from the the oviduct. Spermatozoa from the epididymis were getting and entered on media by pH (7.5, 7.6, 7.7). The faster sperms used for IVF. Embryo of control groups and exprrimental group were transferred into foster mother and after birth, male and female infants were counted. Analysis of data was done by spss, Anova,softwere. Results: The proportion of male and female in control group(pH=7.4), experimental group 1 (pH=7.5) and experimental group2 (pH=7.6) and experimental group3 (pH=7.7) was (48.61), (34.95), (34.35), (33.66) (Mean ± SD) respectively. The comparison of proportion of male to female infant between control group and experimental groups showed significant differences (p <0.05). But there was not significant difference among experiment groups 2, 3 with conrol group. Conclusion: Searches showed that activity of the sperms with X chromosome proportion to Y chromosome in alkaline environments with sodium carbonate increased and chance of female infant birth will increase.