P-95: Effect of Nano Silver on Nitric Oxide Production in Chicken's Sertoli Cells

Background: Chicken sertoli cells are a model to evaluate the effects of toxic Materialss. These cells can actively proliferate in the in vitro conditions and they are able to passage several times. Because of the widespread use of nanosilver in life and also few studies on the effects of these Materialss on different cell lines, evaluation of its toxic effects is felt. Mechanism of nanosilver toxicity is not completely obvious; there are only some reports of its harmful effects. In the present study, an attempt has been done to study the effect of nanosilver on the chicken Sertoli cells in nitric oxide production, lipid per oxidation and antioxidant power. Nitric oxide has a dual role; in low concentrations is considered as a scavenger agent while in high concentrations acts as a free radical. Materials and Methods: Rooster sertoli cells were isolated and cultured, then were exposed to different doses of nanosilver (25, 75 and125 μg/ml). After 48 hours, cells were counted and levels of nitric oxide, TBARS and FRAP were examined. Results: The results indicated that with increasing of nanosilver concentrations, the percentage of living cells was reduced. This reduction of living cells in concentrations of 125 and 75 μg/ml of nanosilver, was significant (p <0.05). In nitric oxide measurement, there was a significant reduction in concentrations of 125 μg/ml of nanosilver with compared to control. It is likely that nitric oxide acted as a scavenger agent. TBARS level was significantly (p<0.05) increased with compared to control. Conclusion: Studies indicated that the antioxidant power was increased but no significant difference was shown. Taken together, it can be noted that large amount of nanosilver induce its toxic effects by producing oxidant compounds in sertoli cells. Nanosilver had no increasing effect on nitric oxide production or possibly oxidants compounds neutralize high concentrations of nitric oxide.