Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses (NDV) isolates, 2010 -2012

نویسندگان

  • Hossein Hossein Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran
  • Masoud Hashemzadeh Department of Research & Production of Veterinary and Poultry Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran
  • Omid Madadgar Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Seyed Ali Ghafouri Department of Poultry Health and Diseases, Iranian Veterinary Organization, Tehran, Iran
  • Seyed Milad Vahedi Students' Scientific Association of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Seyed Sina Bagheri Students' Scientific Association of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Vahid Karimi Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده مقاله:

BACKGROUND: Newcastle disease virus (NDV) is the causativeagent of the Newcastle disease (ND), a highly contagiousdisease in birds that causes significant economic losses to thepoultry industry worldwide. ND is endemic in Iran and outbreaksare reported regularly in commercial poultry flocks and differentspecies of birds. OBJECTIVES:The current study was carried outto characterize NDV based on phosphorprotein (P) gene fromrecent outbreaks in Iran, 2010-2012. METHODS: The P genefragment of NDV isolates of five chickens, 1 ostrich, and 1Pigeon paramyxovirus-1 was obtained by RT-PCR and sequenced.RESULTS: Phylogenetic analysis of sequences revealed thatchicken and ostrich NDV isolates were closely related andplaced in the genotype VII and Pigeon Paramyxovirus-1 waslocated in the genotype V. CONCLUSIONS:This is the first reportof Phosphoprotein gene sequences of NDV strains isolated inIran. This study will help us to understand the epidemiology andmolecular characteristics of Newcastle disease virus in Iran.

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phylogenetic study based on the phosphoprotein gene of iranian newcastle disease viruses (ndv) isolates, 2010 -2012

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عنوان ژورنال

دوره 8  شماره 2

صفحات  73- 77

تاریخ انتشار 2014-08-01

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