S. Saha

Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

[ 1 ] - In vitro Maturation and Fertilization of Buffalo Oocytes Cultured in Media Supplemented with Bovine Serum Albumin

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...

[ 2 ] - Effect of Fresh and Frozen Semen on in vitro Fertilization and Subsequent Development of Goat Embryos

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II sta...

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