Mahnaz Azadpour

Department of Genetics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

[ 1 ] - Recombinant production and affinity purification of the FraC pore forming toxin using hexa-His tag and pET expression cassette

Objective(s): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function. In this study, its coding sequence cloned as a fusion protein with His-tag for simple production and rapid purification. Materials and Methods: After PCR a...

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