Elham Mohit
Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran Protein Technology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
[ 1 ] - Exosomes Secreted by Normoxic and Hypoxic Cardiosphere-derived Cells Have Anti-apoptotic Effect
Cardiosphere-derived cells (CDCs) have emerged as one of the most promising stem cell types for cardiac protection and repair. Exosomes are required for the regenerative effects of human CDCs and mimic the cardioprotective benefits of CDCs such as anti-apoptotic effect in animal myocardial infarction (MI) models. Here we aimed to investigate the anti-apoptotic effect of the hypoxic and normoxic...
[ 2 ] - Exosomes Secreted by Normoxic and Hypoxic Cardiosphere-derived Cells Have Anti-apoptotic Effect
Cardiosphere-derived cells (CDCs) have emerged as one of the most promising stem cell types for cardiac protection and repair. Exosomes are required for the regenerative effects of human CDCs and mimic the cardioprotective benefits of CDCs such as anti-apoptotic effect in animal myocardial infarction (MI) models. Here we aimed to investigate the anti-apoptotic effect of the hypoxic and normoxic...
[ 3 ] - Evaluation of the Effect of Promoter Type on the Immunogenicity of the Live Recombinant Salmonella Vaccines Expressing Escherichia Coli Heat-labile Enterotoxins (LTB)
Enterotoxigenic Escherichia coli (ETEC)-induced diarrhoea is the second most commoncause of death in children in the developing countries. Heat labile toxin (LT) is responsible forETEC-induced diarrhoea. In the present study, a novel live ETEC vaccine based on subunitB of LT (LTB) expression in attenuated PhoPc Salmonella strain was developed. Herein, weaimed to compare the in-vitro activity of...
[ 4 ] - Evaluation of the Effect of Promoter Type on the Immunogenicity of the Live Recombinant Salmonella Vaccines Expressing Escherichia Coli Heat-labile Enterotoxins (LTB)
Enterotoxigenic Escherichia coli (ETEC)-induced diarrhoea is the second most commoncause of death in children in the developing countries. Heat labile toxin (LT) is responsible forETEC-induced diarrhoea. In the present study, a novel live ETEC vaccine based on subunitB of LT (LTB) expression in attenuated PhoPc Salmonella strain was developed. Herein, weaimed to compare the in-vitro activity of...
[ 5 ] - Optimization of HER2-based and cell-based ELISA for detection of trastuzumab biosimilar
Introduction: ELISA is a sensitive, specific, reproducible and fast method to quantify the biological activity of antibodies. Trastuzumab is a humanized monoclonal antibody against HER2 receptors which prevents the initiation of downstream signaling pathway. Trastuzumab can be used as a positive control in the ELISA experiments for anti-HER2 antibodies. Additionaly, insufficient washing and blo...
[ 6 ] - Enhanced Solubility of Anti-HER2 scFv Using Bacterial Pelb Leader Sequence
Single chain Fragment variable (scFv) is an antibody fragment consisting variable regions of heavy and light chains. scFvs enhance their penetrability into tissues while maintaining specific affinity and having low immunogenicity. Insoluble inclusion bodies are formed when scFvs are expressed in reducing bacterial cytoplasm. One strategy for obtaining functionally active scFv is to translocate ...
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