S. D. Kharche

Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India

[ 1 ] - Effects of different activation protocols on cleavage rate and blastocyst production of caprine oocytes

The present study was undertaken to assess the effect of different chemical activators along with 6-DMAP on in vitro matured caprine oocytes. From 4332 ovaries, 14235 cumulus oocyte complexes (COCs) were collected which were matured in TCM-199 medium containing follicle stimulating hormone (FSH) (5 µg/ml), Leutinizing hormone (LH) (10 µg/ml), oestradiol-17β (1 µg/ml) supplemented with 10% fetal...

[ 2 ] - Study of TALP and TRIS citrate medium on caprine sperm capacitation and subsequent in vitro embryo production

The aim of the present investigation was to compare Tyrode’s albumin lactate pyruvate (TALP) medium and TRIS citrate medium for capacitation of caprine sperm. In experiment 1 capacitation was assessed by chlortetracycline assay and in experiment 2 with in vitro fertilization and embryo development. In experiment 2, cumulus oocyte complexes (COCs) recovered by slicing the caprine ovaries were ma...

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