A. Jamshidi

Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

[ 1 ] - Toxigenic Clostridium difficile in retail packed chicken meat and broiler flocks in northeastern Iran

This study was designed to evaluate the occurrence of Clostridium difficile in both broiler chicken farms and packed chicken parts sold at market places in Mashhad, the second most popular Islamic pilgrimage city after Mecca in northeastern Iran. The fresh faecal samples were obtained from broiler farms, while the chicken packs were purchased from retail outlets across the city at market places...

[ 2 ] - Modeling the effects of Bunium persicum (Black Zira) essential oil, pH, inoculums size and temperature on the growth of Listeria monocytogenes

Listeriosis is acknowledged as a major foodborne disease throughout the world caused by Listeria monocytogenes. Different factors can affect the growth of food borne microbial pathogens. The aim of this study was to investigate the combined effects of different concentrations of Bunium persicum essential oil (EO) (0%, 0.08%, 0.16%, 0.24%), three incubation temperatures (35ºC, 25ºC, 4ºC), three ...

[ 3 ] - Isolation and identification of Helicobacter pullorum from caecal content of broiler chickens in Mashhad, Iran

The presence of Helicobacter pullorum in intestinal tract of broiler chickens may be a potential risk for human health. In this study, a total of 100 caecal samples of broilers carcasses from 20 flocks at a poultry abattoir in Mashhad suburb were tested for the presence of H. pullorum using modified conventional culture method by combination of culturing on Brucella sheep blood agar and a filte...

[ 4 ] - Identification of shiga toxin producing Escherichia coli O157:H7 in raw cow milk samples from dairy farms in Mashhad using multiplex PCR assay

In this study 130 bulk tank milk samples which were delivered to the Pegah Pasturisation Factory inMashhad were collected randomly during the summer months. Samples were firstly enriched in modifiedtrypticase soy broth containing novobiocin, followed by plating onto sorbitol MacConkey agar supplementedwith cefixime and potassium tellurite for isolation of Escherichia coli O157:H7. Consequently ...

[ 5 ] - The presence of Listeria monocytogenesin raw milk samples in Mashhad, Iran

The purpose of this preliminary study was to determine the prevalence of raw milk contamination with Listeria monocytogenes. In this study, 100 bulk tank milk samples were collected randomly and delivered to Pegah Pasteurization Factory in Mashhad.For isolation and identification of L. monocytogenes, the samples were first enriched using cold enrichment method in Listeriaenrichment broth, follo...

[ 6 ] - The effect of short-time microwave exposures on Salmonella typhimurium inoculated onto chicken drumettes

  Salmonella     species show different disease syndromes and host specificity, according to their antigenic profile. Salmonella enterica serovar typhimurium is one of the most frequently isolated serovar from foodborne outbreaks. Poultry meat has been identified as one of the principal foodborne sources of Salmonella. In this study, the effect of microwave treatment of chicken meat samples...

[ 7 ] - Growth limits of Staphylococcus aureus as a function of temperature, acetic acid, NaCl concentration, and inoculum level

Staphylococcus aureus is one of the most prevalent causes of gastroenteritis worldwide. Knowing the precise boundary for the growth/no growth interface of S. aureus and also determining the period of time needed for bacterial growth initiation is necessary for food safety risk assessment. This study was designed to examine the combined effects of temperature, acetic acid, inoculum level and NaC...

[ 8 ] - Isolation and identification of Campylobacter spp. and Campylobacter coli from poultry carcasses by conventional culture method and multiplex PCR in Mashhad, Iran

The genus Campylobacter  is of great importance to public health because it includes several species that may cause diarrhoea. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, during the autumn months of 2005, a total of 100 samples from poultry carcasses, representing 20 broiler flocks were obtained by rinse test, after the chilling stage ...

[ 9 ] - Isolation of Escherichia coli O157:H7 from ground beef samples collected from beef markets, using conventional culture and polymerase chain reaction in Mashhad, northeastern Iran

  Escherichia coli O157:H7 is an important human pathogen causing haemorrhagic colitis, haemolyticuraemic syndrom and thrombotic thrombocytopenic purpura. In this study, 100 ground beef samples were collected randomly from beef markets in June 2004. For isolation of the bacteria, samples were firstly enriched in modified trypticase soy broth, followed by plating onto sorbitol MacConkey agar sup...

[ 10 ] - Evaluation of PCR amplification from fixed caecal tissue to determine the infection of experimentally infected laying hens with Brachyspira pilosicoli

Avian intestinal spirochaetosis (AIS) is a condition arising from colonization of the caeca and colon of birds with anaerobic spirochaetes of the genus Brchyspira. The purpose of the present study was to evaluate PCR amplification from formalin-fixed, paraffin-embedded tissue method for detecting infected laying hens with Brachyspira pilosicoli  with the same primers that have been used for det...

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