A. Jolodar
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
[ 1 ] - Identification of a cDNA sequence coding for kruppellike factor 2b (Klf2b) from the skin mucosa of common carp (Cyprinus carpio)
Kruppel-like factors (Klfs) are a highly related zinc-finger family of transcription factors implicated inthe regulation of the eukaryotic cellular growth and differentiation of a diverse set of cells in mammal. UsingRT-PCR technique, a 456 bp cDNA fragment encoding N-terminus part of a Klf2b was isolated from the skinmucosa of common carp (Cyprinus carpio) using two degenerative oligonucleotid...
[ 2 ] - Determination of aspartic protease gene dosage in the Onchocerca volvulusgenome
Aspartic proteases are a relatively small group of enzymes which express in various nematodes including Onchocerca volvulus. An estimation of the gene copy number corresponding to the OV7A clone, which contains a cDNA insert encoding approximately two-thirds of the entire coding sequence of aspartic protease of O. volvulus, was made by slot blot analysis in a closely related species O. gibsonig...
[ 3 ] - PCR screening of the Wolbachia in some arthropods and nematodes in Khuzestan province
Wolbachia is an obligatory, intracellular α-proteobacterium which infect the reproductive and somatic tissues of some arthropod and nematode populations. Because there are not any available data on the presence of this bacterium in Iran, the present study was done to determine the presence of this bacterium among 30 species of arthropods and nematodes. After DNA extraction from samples, we scre...
[ 4 ] - Molecular cloning of adenylate kinase from the human filarial parasite Onchocerca volvulus
Adenylate kinases (ADK) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. In this study, the cloning of a cDNA encoding an adenylate kinase from the filariaOnchocerca volvulus has been described. Using PCR technique, a 281 bp cDNA fragment encoding part ofan adenylate kinase was isolated from an O. volvulus cDNA library. Use of this fragment as a p...
[ 5 ] - Molecular characterization of apolipoprotein A-I from the skin mucosa of Cyprinus carpio
Apolipoprotein A-I is the most abundant protein in Cyprinus carpio plasma that plays an important role in lipid transport and protection of the skin by means of its antimicrobial activity. A 527 bp cDNA fragment encoding C terminus part of apoA-I from the skin mucosa of common carp was isolated using RT-PCR. After GenBank database searching, a partial sequence containing a coding sequence (CDS)...
[ 6 ] - Molecular characterization of apolipoprotein A-I from the skin mucosa of Cyprinus carpio
Apolipoprotein A-I is the most abundant protein in Cyprinus carpio plasma that plays an important role in lipid transport and protection of the skin by means of its antimicrobial activity. A 527 bp cDNA fragment encoding C terminus part of apoA-I from the skin mucosa of common carp was isolated using RT-PCR. After GenBank database searching, a partial sequence containing a coding sequence (CDS)...
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