Sajed Ali
Department of Biotechnology, School of Sciences, University of Management and Technology, Sialkot Campus, Sialkot, Pakistan|Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
[ 1 ] - Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana
Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...
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