Mohammad Hossein Nasr Esfahani
[ 3 ] - P-127: The Effect of Supplementation of In Vitro Culture Medium with FGF2 on Ovine Embryo Development
Background: Fibroblast growth factors are a great family of growth factors that exert variety of functions in vertebrates including cell growth, survival, differentiation and migration. FGFRs are transmembrane tyrosine kinase receptors transmitting FGF signals. Four types of FGFRs (1/2/3/4) exist in vertebrates. FGF2 is one of the FGFs detected in bovine and ovine uterus flushes, supposing that...
[ 6 ] - I-8: Role of Sperm Transcripts in Oocyte Activation, Fertilization and Development
It is well established that in addition to generating the diploid state in the zygote, the sperm also endows the oocyte with paternal centrioles, proteins and RNAs. Although the exact roles of mRNA introduced into the oocyte by the sperm are not completely understood. However, literature background, suggested that RNAs may have a vital role in embryo cleavage and further development. Therefore,...
[ 7 ] - P-5: Assessment of Sperm Chromatin Status in Rat Experimental Varicocele
Background: Varicocele is considered as a common cause of male infertility and can affect spermatogenesis. In addition, it also predisposed sperm to chromatin damage and morphological alternation. Other factors, such as environmental and genetic conditions also predispose sperm to these conditions. To answer this question, we aimed to evaluation of DNA fragmentation and protamine deficiency thr...
[ 8 ] - P-11: Varicocele Improved Semen Quality and Chromatin Integrity in Normozoospermic and Non-Normozoospermic Varicocele Individual
Background: Varicocele is related with poor semen quality and sperm chromatin integrity which possibility decreases the spontaneous pregnancy potential. Some of the varicocele individuals have normal semen parameters with failed pregnancy. It is also one of the most controversial issues in the field ofinfertility, especially regarding why, when and for whom varicocelectomy should be implemented...
[ 9 ] - P-58: Which Novel Sperm Selection is Suitable for ICSI? MACS-DGC or DGC-MACS
Background: Super-paramagnetic annexin-V conjugated micro-beads are able to eliminate sperm with phosphatidylserine externalization (PSE). PSE is considered as apoptotic sperm. PES positive sperm can be removed from sperm preparations sample to enhance sperm quality for assisted medical procreation. Therefore, the efficiency of this technique over normal sperm processing procedure such as densi...
[ 10 ] - P-63: Comparison of The Efficacy of Two Culture Media (Handmade vs. Commercial) on In Vitro Development of Vitrified and Non-Vitrified Mouse Embryos
Background: While assisted reproductive techniques (ARTs) are considered to be safe, many concerns exist about the safety of these techniques. Two important and indispensable parts of these techniques are in vitro culture and vitrification. While much progress has been made in developing culture conditions, it remains suboptimal. In order to investigate the effect of culture medium on in vitro ...
[ 11 ] - P-77: HOST Patterns and Sperm Quality
Background: Following the expansion of human infertility, different procedures have been utilized for separation of normal sperm during ICSI (Intra cytoplasmic sperm injection). Hypo-osmotic swelling test (HOST) is one of the tests which have been introduced for assessment of integrity of sperm membrane when confronted with hypo osmotic condition. This test may also reflect intracellular organe...
[ 12 ] - P-82: Effect of SCNT Steps on Relative mRNA Abundances of Sheep Oocytes
Background: The oocyte is a unique cell committed to reprogram fertilizing sperm and to support early stages of embryonic development until the species-specific stage of zygote genome activation that occurs around the second to third cell cycle in sheep embryos. In this sense, considering the huge list of oocyte transcripts, we selected some candidates genes based on their roles of regulating di...
[ 13 ] - P-83: Cloned Sheep Blastocysts Derived from Oocytes Enucleated Manually Using A Pulled Pasteur Pipette
Background: The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulation skill and expensive equipment. Here, we have devel oped an efficient...
[ 14 ] - P-97: Optimized Combined Electrical-Chemical Parthenogenetic Activation for Zona Free Sheep Oocyte
Background: Artificial oocyte activation (AOA) has important implications in assisted reproductive technique (ART) with particular importance in somatic cell nuclear transfer (SCNT). Parthenogenesis activation using zona- free oocytes offers an alternative model that could be applied to develop protocols for the activation of reconstructed embryos for cloning. Materials and Methods: To establis...
[ 15 ] - P-108: Effect of Phosphodiesterase Type 3 Inhibitor on Nuclear Maturation and In Vitro Development of Ovine Oocytes
Background: Present study aims to investigate if PMC of ovine oocytes in the presence of cilostamide a phosphodiesterase type 3 (PDE3) inhibitor can improve the shortcomings of conventional IVM system. Materials and Methods: Immature ovine oocytes were retrieved from pre antral follicles of abattoir ovaries by aspiration and after washing in Htcm + %10 FBS were immediately classified in four gr...
[ 16 ] - P-109: Using SMAD2/3 Inhibitor to Investigate the Importance of GDF9 Signaling on Ovine Cumulus Expansion and Subsequent Embryonic Development
Background: Oocyte in vitro maturation success rate is still low in compareision to oocyte in vivo maturation. Thus prediction and improvement of oocyte competency are two critical issues in assisted reproductive technology. New insights into oocyte-cumulus cell regulatory loop may assist our optimization of in vitro maturation technique. Maturation and developmental competency of cumulus oocyt...
[ 17 ] - P-114: Gene
[ 18 ] - P-124: Association between Fertilization Rate and Externilization of Phosphatidyl Serine
Background: Externalization of PS (EPS) is considered as a sign of early apoptosis. In addition, EPS also occurs during capacitation and the acrosome reaction. During this study we aimed to evaluate the relationship between EPS and fertilization, embryo quality and pregnancy outcomes in couples who were candidates for ICSI. Materials and Methods: Semen samples (n = 43) were collected from infer...
[ 19 ] - P-197: Aberrant Gene Expression Profile in Blastocyst Embryos As Consequences of Assisted Reproductive Technologies
Background: Currently, ARTs are of indispensable importance in treatment of human infertility. While ARTs are considered to be safe, some studies suggest that ARTs manipulations may perturb the epigenetic information and subsequently the health of the offspring. To examine these events further, this study was conducted to assess the effect of superovulation, in vitro culture and vitrification o...
[ 23 ] - I-5: Fifteen Years after Dolly: The Perspectives on Cellular Reprogramming
s:1202:"It is a truly amazing time to developmental biology. During recent decades, three important breakthroughs have been developed: (i) isolation of stem cells from embryo, (ii) animal cloning by nuclear transfer (NT), and (iii) and induced pluripotent stem cells (iPS). Considering these three approaches of "Cellular Reprogramming", it seems that the required elements for cell therapy now ex...
[ 27 ] - Flow Cytometry: A New Approach for Indirect Assessment of Sperm Protamine Deficiency
Background Flow cytometry (FCM) has been extensively used to study mammalian sperm in the areas of clinical andrology and reproductive toxicology. FCM provides a powerful advantage over microscopy technique in terms of rapid accurate and reproducible technology for the quantification of various cell characteristics including chromatin status. During spermiogenesis histones are replaced by prota...
[ 28 ] - In Vitro Comparison of Soybean Lecithin Based-Extender with Commercially Available Extender for Ram Semen Cryopreservation
Background Egg yolk is the main cryoprotectant agent in semen freezing extenders which is used in order to protect spermatozoa against cold shock. However elimination of animal bioproducts from the cryopreservation protocol is becoming mandatory. Therefore the aim of this study is to compare a previously studied homemade soya bean lecithin based extender with a commercially available extender f...
[ 29 ] - Highly Efficient In Vitro Production of Bovine Blastocyst in Cell-Free Sequential Synthetic Oviductal Fluid vs. TCM199 Vero Cell Co-Culture System
Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...
[ 30 ] - Etiology and Evaluation of Sperm Chromatin Anomalies
Evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. Routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear DNA integrity of spermatozoa. It has been suggested that altered nuclear chromatin struc...
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